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Interaction of SH1-blocked HMM with Actin and ATP

Nature New Biology volume 240, pages 207–208 (1972)Cite this article

Abstract

NUMEROUS studies have been performed on the effect of blocking the SH groups of myosin with sulphydryl reagents1–8. In particular, the effect of NEM has become quite clear; on blocking one specific SH group (referred to as SH1) per myosin subunit, the EDTA-ATPase of the myosin is completely inhibited whereas the Ca-ATPase is markedly activated9. However, the interaction of SH1-blocked myosin and actin has not been as thoroughly investigated. Sekine and Yanoguchi have reported that blocking SH1 with NEM does not affect the ATPase activity and superprecipitation of actomyosin10. On the other hand, the effect of native tropomyosin on the actomyosin ATPase has been reported to be abolished by NEM under certain conditions although differences were reported depending on whether myosin or actomyosin was exposed to the NEM11.

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Authors and Affiliations

  1. Section on Cellular Physiology, Laboratory of Biochemistry, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland, 20014

    R. SILVERMAN, E. EISENBERG & W. W. KIELLEY

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  1. R. SILVERMAN
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  2. E. EISENBERG
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  3. W. W. KIELLEY
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SILVERMAN, R., EISENBERG, E. & KIELLEY, W. Interaction of SH1-blocked HMM with Actin and ATP. Nature New Biology 240, 207–208 (1972). https://doi.org/10.1038/newbio240207a0

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