Abstract
Until recently, it has not been possible unequivocally to demonstrate that steroid hormones induce increases in the quantity of mRNA in animal cells; proof would require the isolation of a specific mRNA, its unambiguous translation, and the demonstration that the intracellular concentration of this specific mRNA in target tissue is strictly dependent on the state of hormonal stimulation. Here we report studies of progesterone-mediated induction of mRNA in the chick oviduct. In this tissue, both oestrogen and progesterone selectively induce the synthesis of distinct proteins1–10. Progesterone induces de novo synthesis of the specific protein avidin in oestrogen-primed oviduct and the specificity and kinetics of induction of this protein have been investigated1,2,11–14. We have shown that progesterone causes major quantitative and qualitative alterations in oviduct gene transcription1,12,15,16, but this is only indirect evidence that any progesterone-mediated change in specific mRNAs occurs in oviduct target cells. We wished to test directly the production of a specific mRNA as the result of progesterone administration.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
O'Malley, B. W., McGuire, W. L., Kohler, P. O., and Korenman, S. G., Recent Prog. Hormone Res., 25, 105 (1969).
O'Malley, B. W., Biochemistry, 6, 2546 (1967).
Korenman, S. G., and O'Malley, B. W., Endocrinology, 83, 11 (1968).
Kohler, P. O., Grimley, P. M., and O'Malley, B. W., J. Cell Biol., 40, 8 (1969).
Oka, R., and Schimke, R. T., J. Cell Biol., 43, 123 (1969).
Kohler, P. O., Grimley, P. M., and O'Malley, B. W., Science, 160, 86 (1968).
O'Malley, B. W., McGuire, W. L., and Korenman, S. G., Biochim. Biophys. Acta, 145, 204 (1967).
Rhoads, R. E., McKnight, G. S., and Schimke, R. T., J. Biol. Chem., 246, 7407 (1971).
Means, A. R., Comstock, J. P., Rosenfeld, G. C., and O'Malley, B. W., Proc. US Nat. Acad. Sci., 69, 1146 (1972).
Rosenfeld, G. C., Comstock, J. P., Means, A. R., and O'Malley, B. W., Biochem. Biophys. Res. Commun., 46, 1695 (1972).
O'Malley, B. W., and Kohler, P. O., Proc. US Nat. Acad. Sci., 58 (1967).
O'Malley, B. W., and McGuire, W. L., J. Clin. Invest., 47, 654 (1968).
O'Malley, B. W., McGuire, W. L., and Middleton, P. A., Endocrinology, 81, 677 (1967).
O'Malley, B. W., Spelsberg, T. C., Schrader, W. T., Chytil, F., and Steggles, A. W., Nature, 235, 141 (1972).
O'Malley, B. W., and McGuire, W. L., Endocrinology, 84, 63 (1969).
Hahn, W. E., Church, R. B., Gorbman, A., and Wilmot, L., Gen. Comp. Endocrinol., 11 (1968).
Brawerman, G., Biochim. Biophys. Acta, 76, 322 (1963).
Brawerman, G., Mendecki, J., and Lee, S. Y., Biochemistry, 11, 637 (1972).
Rosenfeld, G. C., Comstock, J. P., Means, A. R., and O'Malley, B. W., Biochem. Biophys. Res. Commun., 47, 387 (1972).
Gilbert, J. M., and Anderson, W. F., J. Biol. Chem., 245, 2342 (1970).
Stavnezer, J., and Huang, R. C., Nature, 230, 172 (1971).
O'Malley, B. W., and Korenman, S. G., Life Sci., 6, 1953 (1967).
Korenman, S. G., and O'Malley, B. W., Biochim. Biophys. Acta, 140, 174 (1967).
Comstock, J. P., Rosenfeld, G. C., O'Malley, B. W., and Means, A. R., Proc. US Nat. Acad. Sci. (in the press).
Means, A. R., Abrass, I. B., and O'Malley, B. W., Biochemistry, 10, 1561 (1971).
Obrig, T., Irvin, J., Culp, W., and Hardesty, B., Eur. J. Biochem., 21, 31 (1971).
Stewart, M. L., Grollman, A. P., and Huang, M., Proc. US Nat. Acad. Sci., 68, 97 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
O'MALLEY, B., ROSENFELD, G., COMSTOCK, J. et al. Steroid Hormone Induction of a Specific Translatable Messenger RNA. Nature New Biology 240, 45–48 (1972). https://doi.org/10.1038/newbio240045a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/newbio240045a0
This article is cited by
-
Effect of actinomycin-D on the progesterone induced cytodifferentiation of the chick oviduct epithelium
Histochemistry (1978)
-
Poly ADP-ribosylation of DNA-dependent RNA polymerase I from quail oviduct. Dependence on progesterone stimulation.
Molecular and Cellular Biochemistry (1976)
-
Estrogen induction of ovalbumin mRNA: Evidence for transcription control
Molecular and Cellular Biochemistry (1975)
-
Specificity of the Control of Protein Synthesis by Haemin
Nature New Biology (1973)