E. coli DNA Polymerase I and other Host Functions participate in T4 DNA Replication and Recombination

Abstract

The recognition of bacterial functions involved in DNA metabolism of bacteriophage T4 might reveal interactions between different enzymes during DNA replication and recombination. To detect such functions we have studied the replication of complete and incomplete T4 chromosomes in various mutant strains of Escherichia coli that are defective in their own DNA metabolism. We found that several E. coli functions can substitute for phage functions in T4 replication and recombination, and will discuss here the role of the E. coli pol A gene which codes for DNA polymerase I1–4 and of the dna B and E genes3,5.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    De Lucia, P., and Cairns, J., Nature, 224, 1164 (1969).

  2. 2

    Kornberg, A., Science, 163, 1410 (1969).

  3. 3

    Gross, J. D., in Current Topics in Microbiology and Immunology, 57, 39 (Springer, Berlin, Heidelberg, New York, 1972).

  4. 4

    Monk, M., and Kinross, J., J. Bact., 109, 971 (1972).

  5. 5

    Wechsler, J. A., Second Annual Steenbock Symp. (in the press).

  6. 6

    Mosig, G., Genetics, 59, 137 (1968).

  7. 7

    Mosig, G., Carnighan, J. R., Bibring, J. B., Cole, R., Bock, H.-G. O., and Bock, S., J. Virol., 9, 857 (1972).

  8. 8

    Mosig, G., and Werner, R., Proc. US Nat. Acad. Sci., 64, 747 (1969).

  9. 9

    Marsh, R. C., Breschkin, A. M., and Mosig, G., J. Mol. Biol., 60, 213 (1971).

  10. 10

    Mosig, G., J. Mol. Biol., 53, 503 (1970).

  11. 11

    Mosig, G., Ehring, R., Schliewen, W., and Bock, S., Mol. Gen. Genetics, 113, 51 (1971).

  12. 12

    Kozinski, Andrzej W., Kozinski, P. B., and James, R., J. Virol., 1, 758 (1967).

  13. 13

    Werner, R., Cold Spring Harbor Symp. Quant. Biol., 33, 501 (1968).

  14. 14

    Kuempel, P. L., and Veomett, G. E., Biochem. Biophys. Pes. Commun., 41, 973 (1970).

  15. 15

    Okazaki, R., Arisawa, M., and Sugino, A., Proc. US Nat. Acad. Sci., 68, 2954 (1971).

  16. 16

    Kelly, R. B., Atkinson, M. R., Huberman, J. A., and Kornberg, A., Nature, 224, 495 (1969).

  17. 17

    Setlow, P., and Kornberg, A., J. Biol. Chem., 247, 232 (1972).

  18. 18

    Mosig, G., Adv. Genetics, 15, 1 (1970).

  19. 19

    Broker, T. R., and Lehman, I. R., J. Mol. Biol., 60, 131 (1971).

  20. 20

    Lehman, I. R., Huang, W. M., and Chien, J. R., Second Annual Steenbock Symp. (in the press).

  21. 21

    Doermann, A. H., and Boehner, L., Virology, 21, 551 (1963).

  22. 22

    Womack, F. C., Virology, 21, 232 (1963).

  23. 23

    De Waard, A., Paul, A. V., and Lehman, I. R., Proc. US Nat. Acad. Sci., 54, 1241 (1965).

  24. 24

    Warner, H. R., and Barnes, J. E., Virology, 28, 100 (1966).

  25. 25

    Epstein, R. H., Bolle, A., Steinberg, C. M., Kellenberger, E., Boy De La Tour, E., Chevalley, R., Edgar, R. S., Susman, M., Denhardt, G. H., and Lielausis, A., Cold Spring Harbor Symp. Quant. Biol., 28, 375 (1963).

  26. 26

    Werner, R., Cold Spring Harbor Symp. Quant. Biol., 33, 501 (1968).

  27. 27

    Yegian, C. D., Mueller, M., Selzer, G., Russo, V., and Stahl, F. W., Virology, 46, 900 (1971).

  28. 28

    Majumdar, C., Dewey, M., and Frankel, F. R., Virology, 49, 134 (1972).

  29. 29

    Wickner, W., Brutlag, D., Schekman, R., and Kornberg, A., Proc. US Nat. Acad. Sci., 69, 965 (1972).

  30. 30

    Keller, W., Proc. US Nat. Acad. Sci., 69, 1560 (1972).

  31. 31

    Sugino, A., Hirose, S., and Okazaki, R., Proc. US Nat. Acad. Sci., 69, 1863 (1972).

  32. 32

    Zissler, J., Signer, E., and Schaefer, F., in The Bacteriophage Lambda (edit. by Hershey, A. D.), 469 (Cold Spring Harbor Press, New York, 1971).

  33. 33

    Zissler, J., Signer, E., and Schaefer, F., in The Bacteriophage Lambda (edit. by Hershey, A. D.), 455 (Cold Spring Harbor Press, New York, 1971).

  34. 34

    Kingsbury, D. T., and Helinski, D., Biochem. Biophys. Res. Commun., 41, 1538 (1970).

  35. 35

    Goebel, W., Nature New Biology, 231, 67 (1972).

  36. 36

    Gefter, M. L., Hirota, Y., Kornberg, T., Wechsler, J. A., and Barnoux, C., Proc. US Nat. Acad. Sci., 68, 3150 (1971).

  37. 37

    Nüsslein, V., Otto, B., Bonhoeffer, F., and Schaller, H., Nature New Biology, 234, 285 (1971).

  38. 38

    Georgopoulos, C. P., and Herskowitz, I., in The Bacteriophage Lambda (edit. by Hershey, A. D.), 553 (Cold Spring Harbor Press, New York, 1971).

  39. 39

    Delius, H., Howe, C., and Kozinski, A., Proc. US Nat. Acad. Sci., 68, 3049 (1971).

  40. 40

    Adams, M. H., Bacteriophages (Interscience, New York, 1959).

  41. 41

    Lanka, E., and Schuster, H., Mol. Gen. Genetics, 106, 274 (1970).

  42. 42

    Wechsler, J. A., and Gross, J. D., Mol. Gen. Genetics, 113, 273 (1971).

  43. 43

    Carl, P. L., Mol. Gen. Genetics, 109, 107 (1970).

  44. 44

    Schaller, H., Otto, B., Nüsslein, V., Huf, J., Herrman, R., and Bonhoeffer, F., J. Mol. Biol., 63, 183 (1972).

  45. 45

    Vosberg, H. P., and Hoffmann-Berling, H., J. Mol. Biol., 58, 739 (1971).

  46. 46

    Fangmann, W. L., and Feiss, M., J. Mol. Biol., 44, 103 (1969).

  47. 47

    Hershey, A. D., and Mosig, G., Carnegie Institution Year Book, 64, 521 (Carnegie Institution, Washington, DC, 1965).

  48. 48

    Fuchs, J. A., Karlström, H. O., Warner, H. R., and Reichard, P., Nature New Biology, 238, 69 (1972).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading