Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letters to Editor
  • Published:

Properties of Reconstituted Chromatin and Nucleohistone Complexes

Nature New Biology volume 239, pages 134–135 (1972)Cite this article

Abstract

WE have prepared complexes of calf thymus DNA with whole histone, with individual histone fractions and with whole histone and acidic chromosomal proteins from the same tissue by taking up the components in 5 M urea, 2 M NaCl and gradually removing the urea and salt1. These nudeoproteins were examined in a “Philips EM 300” electron microscope after phosphotungstate-uranyl acetate staining2; their template activities for RNA synthesis by Escherichia coli DNA-dependent RNA polymerase were compared; their protein3 and DNA4 contents were measured and the fraction of total DNA phosphate groups free to bind basic dye5 was estimated.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Paul, J., & Gilmour, R. S., J. Mol. Biol., 34, 305 (1968).

    Article  CAS  Google Scholar 

  2. Miller, O. L., Hamkalo, B. A., & Thomas, C. A., Science, 169, 392 (1970).

    Article  Google Scholar 

  3. Itzhaki, R. F., & Gill, A. M., Anal. Biochem., 9, 401 (1964).

    Article  CAS  Google Scholar 

  4. Burton, K., Biochem. J., 62, 315 (1956).

    Article  CAS  Google Scholar 

  5. Miura, A., & Ohba, Y., Biochim. Biophys. Acta, 145, 436 (1967).

    Article  CAS  Google Scholar 

  6. Johns, E. W., Biochem. J., 92, 55 (1964).

    Article  CAS  Google Scholar 

  7. Johns, E. W., Biochem. J., 105, 611 (1967).

    Article  CAS  Google Scholar 

  8. Dick, C., & Johns, E. W., Biochim. Biophys. Acta, 174, 380 (1969).

    Article  CAS  Google Scholar 

  9. MacGillivray, A. J., Carroll, D., & Paul, J., FEBS Letters, 13, 204 (1971).

    Article  CAS  Google Scholar 

  10. Wang, T. Y., Exp. Cell Res., 61, 455 (1970).

    Article  CAS  Google Scholar 

  11. Itzhaki, R. F., Biochem. J., 122, 583 (1971).

    Article  CAS  Google Scholar 

  12. Clark, R. J., & Felsenfeld, G., Nature New Biology, 229, 101 (1971).

    Article  CAS  Google Scholar 

  13. Phillips, D. M. P., Experientia, 24, 668 (1968).

    Article  CAS  Google Scholar 

  14. Richards, B. M., & Pardon, J. F., Exp. Cell Res., 62, 184 (1970).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Beatson Institute for Cancer Research, Glasgow

    J. PAUL & I. R. MORE

Authors
  1. J. PAUL
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. R. MORE
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

PAUL, J., MORE, I. Properties of Reconstituted Chromatin and Nucleohistone Complexes. Nature New Biology 239, 134–135 (1972). https://doi.org/10.1038/newbio239134a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio239134a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing