Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Topology of DNA and N Gene Expression in Coliphage Lambda

Abstract

In a transformation system designed to assay gene function from the infecting DNA we find that the intact λ molecule is necessary to provide N function, whereas the phage recombination (red) genes can function from half as well as whole λ molecules. Our results may reflect the need of a circular template for efficient N gene transcription in vivo.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Young, E., II., and Sinsheimer, R., J. Mol. Biol., 10, 562 (1964).

    Article  CAS  Google Scholar 

  2. Bode, V. C., and Kaiser, A. D., J. Mol. Biol., 14, 399 (1965).

    Article  CAS  Google Scholar 

  3. Campbell, A., Advan. Genetics, 11, 45 (1962).

    Google Scholar 

  4. Tomizawa, J., and Ogawa, T., Cold Spring Harbor Symp. Quant. Biol., 33, 533 (1968).

    Article  CAS  Google Scholar 

  5. Schnös, M., and Inman, R. B., J. Mol. Biol., 51, 61 (1970).

    Article  Google Scholar 

  6. Herskowitz, I., and Signer, E. R., J. Mol. Biol., 47, 545 (1970).

    Article  CAS  Google Scholar 

  7. Kaiser, A. D., and Hogness, D. S., J. Mol. Biol., 2, 392 (1960).

    Article  CAS  Google Scholar 

  8. Hershey, A. D., Burgi, E., and Ingraham, L., Proc. US Nat. Acad. Sci., 48, 748 (1963).

    Article  Google Scholar 

  9. Hershey, A. D., and Burgi, E., Proc. US Nat. Acad. Sci., 53, 325 (1965).

    Article  CAS  Google Scholar 

  10. Kaiser, A. D., J. Mol. Biol., 4, 275 (1962).

    Article  CAS  Google Scholar 

  11. Davidson, N., and Szybalski, W., The Bacteriophage Lambda (edit. by Hershey, A.), 45 (Cold Spring Harbor Press, New York, 1971).

    Google Scholar 

  12. Signer, E., Echols, H., Weil, J., Radding, C., Schulman, M., Moore, L., and Manly, K., Cold Spring Harbor Symp. Quant. Biol., 33, 711 (1968).

    Article  CAS  Google Scholar 

  13. Radding, C. M., and Echols, H., Proc. US Nat. Acad. Sci., 60, 707 (1968).

    Article  CAS  Google Scholar 

  14. Kumar, S., Bovre, K., Guha, A., Hradecna, Z., Maher, sr., V. M., and Szybalski, W., Nature, 221, 823 (1969).

    Article  CAS  Google Scholar 

  15. Thomas, K., J. Mol. Biol., 49, 393 (1970).

    Article  CAS  Google Scholar 

  16. Luzzati, D., J. Mol. Biol., 49, 515 (1970).

    Article  CAS  Google Scholar 

  17. Adler, J., and Templeton, B., J. Mol. Biol., 7, 710 (1963).

    Article  CAS  Google Scholar 

  18. Barbour, S. D., and Clarke, A. J., Proc. US Nat. Acad. Sci., 65, 995 (1970).

    Article  Google Scholar 

  19. Dubnau, E., and Maas, W. K., Molec. Gen. Genetics, 103, 205 (1969).

    Article  Google Scholar 

  20. Unger, R. C., and Clark, A. J., J. Mol. Biol., (in the press).

  21. Hayashi, M., Hayashi, M. N., and Spiegelman, S., Proc. US Nat. Acad. Sci., 51, 351 (1964).

    Article  CAS  Google Scholar 

  22. Warnaar, S. O., Muldder, G., van der Sigtenhorst-van der Sluis, I., Van Kersteren, L. W., and Cohen, J. A., Biochim. Biophys. Acta, 174, 239 (1969).

    Article  CAS  Google Scholar 

  23. Westphal, H., J. Mol. Biol., 50, 407 (1970).

    Article  CAS  Google Scholar 

  24. Cohen, S. N., Maitra, U., and Hurwitz, J., J. Mol. Biol., 26, 19 (1967).

    Article  CAS  Google Scholar 

  25. Roberts, J. W., Nature, 223, 480 (1969).

    Article  CAS  Google Scholar 

  26. Cohen, S. N., and Hurwitz, J., J. Mol. Biol., 58, 635 (1971).

    Article  CAS  Google Scholar 

  27. Franklin, N. C., in The Bacteriophage Lambda (edit. by Hershey, A.), 621 (Cold Spring Harbor Press, New York, 1971).

    Google Scholar 

  28. Egan, J. B., and Hogness, D. S., J. Mol. Biol. (in the press).

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

PILARSKI, L., EGAN, J. Topology of DNA and N Gene Expression in Coliphage Lambda. Nature New Biology 237, 102–104 (1972). https://doi.org/10.1038/newbio237102a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio237102a0

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing