Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letters to Editor
  • Published:

Glucagon Affinity Absorbents: Selective Binding of Receptors of Liver Cell Membranes

Nature New Biology volume 234, pages 268–270 (1971)Cite this article

A Corrigendum to this article was published on 26 January 1972

Abstract

AFFINITY chromatography has been used in the rapid isolation of enzymes, antibodies, antigens and other ligand-binding proteins1–6. Selective adsorbents with biological specificity perhaps may best be used in the resolution and isolation of complex biological structures and important regulatory macromolecules present in cells in very low amounts. For example, polypeptide and steroid hormone receptors, drug receptors, transport proteins and repressor molecules may be well suited for study by this technique because they display specific binding functions with a high degree of affinity.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Cuatrecasas, P., Wilchek, M., and Anfinsen, C. B., Proc. US Nat. Acad. Sci., 61, 636 (1968).

    Article  CAS  Google Scholar 

  2. Cuatrecasas, P., J. Biol. Chem., 245, 3059 (1970).

    CAS  PubMed  Google Scholar 

  3. Cuatrecasas, P., Nature, 228, 1327 (1970).

    Article  CAS  Google Scholar 

  4. Cuatrecasas, P., and Anfinsen, C. B., Ann. Rev. Biochem., 40, 259 (1971).

    Article  CAS  Google Scholar 

  5. Cuatrecasas, P., in Biochemical Aspects of Solid State Chemistry (edit, by Stark, G. R.) (Academic Press, New York, in the press).

  6. Cuatrecasas, P., Adv. in Enzymology, 35 (in the press).

  7. Cuatrecasas, P., Proc. US Nat. Acad. Sci., 63, 450 (1969).

    Article  CAS  Google Scholar 

  8. Turkington, R. W., Biochem. Biophys. Res. Commun., 41, 1362 (1970).

    Article  CAS  Google Scholar 

  9. Selinger, R. C. L., and Civen, M., Biochem. Biophys. Res. Commun., 43, 793 (1971).

    Article  CAS  Google Scholar 

  10. Pohl, S. L., Birnbaumer, L., and Rodbell, M., J. Biol. Chem., 246, 1849 (1971).

    CAS  PubMed  Google Scholar 

  11. Rodbell, M., Krans, H. M. J., Pohl, S. L., and Birnbaumer, L., J. Biol. Chem., 246, 1861 (1971).

    CAS  Google Scholar 

  12. Steers, E., Cuatrecasas, P., and Pollard, H. B., J. Biol. Chem., 246, 196 (1971).

    CAS  PubMed  Google Scholar 

  13. Cuatrecasas, P., Desbuquois, B., and Krug, F., Biochem. Biophys. Res. Commun., 44, 333 (1971).

    Article  CAS  Google Scholar 

  14. Hunter, W. J., and Greenwood, F. C., Nature, 194, 495 (1962).

    Article  CAS  Google Scholar 

  15. Yalow, R. S., and Berson, S. A., Nature, 212, 357 (1966).

    Article  CAS  Google Scholar 

  16. Cuatrecasas, P., Proc. US Nat. Acad. Sci., 68, 1264 (1971).

    Article  CAS  Google Scholar 

  17. Krishna, G., Weiss, B., and Brodie, B. B., J. Pharmacol. Exp. Ther., 163, 379 (1968).

    CAS  Google Scholar 

  18. Rodbell, M., Birnbaumer, L., Pohl, S. L., and Sundby, F., Proc. US Nat. Acad. Sci., 68, 909 (1971).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Medicine, Pharmacology and Experimental Therapeutics, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205

    FOLKER KRUG, BERNARD DESBUQUOIS & PEDRO CUATRECASAS

Authors
  1. FOLKER KRUG
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. BERNARD DESBUQUOIS
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. PEDRO CUATRECASAS
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

KRUG, F., DESBUQUOIS, B. & CUATRECASAS, P. Glucagon Affinity Absorbents: Selective Binding of Receptors of Liver Cell Membranes. Nature New Biology 234, 268–270 (1971). https://doi.org/10.1038/newbio234268a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio234268a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing