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Role of GTP in the Positioning of Formylmethionyl-tRNAf on the E. coli Ribosome

Nature New Biology volume 234, pages 216–218 (1971)Cite this article

Abstract

FORMATION of the E. coli initiation complex between ribosomal subunits, natural messenger RNA and formyl-methionyl-tRNAf (fMet-tRNAf) requires the presence of initiation factors and GTP1–3. In the binding reaction, GTP can be replaced by an analogue, guanylyl-5′-methylene-diphosphonate (GMP-PCP), but the complex does not then react with puro-mycin. Hydrolysis of GTP is therefore required for the formation of an active initiation complex able to carry out peptide bond formation4.

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  1. Institutè of Biochemistry, University of Vienna, Vienna, 1090

    ERNST KUECHLER

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  1. ERNST KUECHLER
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KUECHLER, E. Role of GTP in the Positioning of Formylmethionyl-tRNAf on the E. coli Ribosome. Nature New Biology 234, 216–218 (1971). https://doi.org/10.1038/newbio234216a0

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  • DOI: https://doi.org/10.1038/newbio234216a0

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