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Estimation of Molecular Weights of Protein-SDS Complexes

Abstract

PROTEIN aggregates are often dissociated by sodium dodecyl sulphate (SDS) and the subunit molecular weight then estimated by gel-electrophoresis1–3. This method uses internal standards and at the lower range of molecular weights (approximately 10,000) it may be difficult to find well defined standards. For these proteins it is necessary to utilize the analytical ultracentrifuge.

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References

  1. Reynolds, J. A., and Tanford, C., J. Biol. Chem., 245, 5161 (1970).

    CAS  PubMed  Google Scholar 

  2. Weber, K., and Osborne, M., J. Biol. Chem., 244, 4406 (1969).

    CAS  PubMed  Google Scholar 

  3. Dunker, A. K., and Rueckert, R. R., J. Biol. Chem., 244, 5074 (1969).

    CAS  PubMed  Google Scholar 

  4. Hersh, R. T., and Schachman, H. K., Virology, 6, 234 (1958).

    Article  CAS  PubMed  Google Scholar 

  5. Carusi, E. A., and Sinsheimer, R. L., J. Mol. Biol., 7, 388 (1963).

    Article  CAS  PubMed  Google Scholar 

  6. Spragg, S. P., Anal. Chim. Acta, 38, 137 (1967).

    Article  CAS  PubMed  Google Scholar 

  7. Goodman, R. F., and Spragg, S. P., Ann. NY Acad. Sci., 164, 294 (1969).

    Article  Google Scholar 

  8. Theorell, H., Biochem., 268, 46 (1934).

    CAS  Google Scholar 

  9. Antonini, E., Phys. Rev., 45, 1 (1965).

    Google Scholar 

  10. Knippers, R., and Hoffman-Berling, H., J. Mol. Biol., 21, 281 (1966).

    Article  CAS  PubMed  Google Scholar 

  11. DeGroot, K., Hoenders, H. J., Gerding, J. J. T., and Bloemetidal, H., Biochim. Biophys. Acta, 207, 202 (1970).

    Article  CAS  Google Scholar 

Download references

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BARNETT, W., SPRAGG, S. Estimation of Molecular Weights of Protein-SDS Complexes. Nature New Biology 234, 191–192 (1971). https://doi.org/10.1038/newbio234191a0

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