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Defective DNA Synthesis in Permeabilized Yeast Mutants

Nature New Biology volume 234, pages 171–172 (1971)Cite this article

Abstract

THE simple eukaryote, Saccharomyces cerevisiae, is suitable for combined genetic and biochemical analysis of the cell division cycle. More than forty temperature-sensitive mutants of S. cerevisiae defective in fifteen genes that control various steps of the yeast cell cycle have been detected by screening a collection of mutants with time-lapse photomicroscopy1. Mutations in two genes, cdc4 and cdc8, result in defective DNA synthesis at the restrictive temperature2. The product of cdc8 is apparently required throughout the period of DNA synthesis, because if a strain defective in this gene is shifted to 36° C within the S period, DNA replication ceases. In contrast, the product of cdc4 is apparently required only at the initiation of DNA synthesis because when a strain carrying a defect in this gene is shifted to 36° C, DNA replication already in progress is not impaired. Cells defective in cdc4, however, fail to initiate new rounds of DNA synthesis at the restrictive temperature. Based on these observations the DNA mutants have been tentatively classified as defective in DNA replication (cdc8) and in the initiation of DNA synthesis (cdc4).

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Author notes

  1. LYNNA M. HEREFORD

    Present address: Department of Genetics, University of Washington, Seattle, Washington

Authors and Affiliations

  1. Department of Microbiology, Yale University, New Haven, Connecticut

    LYNNA M. HEREFORD

  2. Department of Genetics, University of Washington, Seattle, Washington

    LELAND H. HARTWELL

Authors
  1. LYNNA M. HEREFORD
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  2. LELAND H. HARTWELL
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HEREFORD, L., HARTWELL, L. Defective DNA Synthesis in Permeabilized Yeast Mutants. Nature New Biology 234, 171–172 (1971). https://doi.org/10.1038/newbio234171a0

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