Abstract
THE availability of homogeneous populations of human and murine myeloma cells has provided a unique opportunity for investigating the mechanism of immunoglobulin formation1. Continuous lines of cultured lymphoid cells producing specific antibody or manifesting delayed hypersensitivity would be even more useful in studying the molecular events of the immune response. Human lymphoid cell lines have been established in long term culture using Epstein–Barr virus (EBV)2, 3 or phyto-haemagglutinin4 but antigen alone has not been effective5. The purpose of the work reported here was selectively to establish antigen-sensitive cells in culture by stimulating peripheral white cells from delayed hypersensitive donors with antigen in vitro and then exposing the cells to EBV. This combination of antigen and virus was chosen because of the following considerations: (1) some RNA and DNA viruses do not replicate in resting lymphocytes but can infect antigen-sensitive lymphocytes which have been stimulated in vitro with mitogens or specific antigen6, 7; (2) polyoma virus transforms cells in the G2 phase of the cell cycle more effectively than in G1 (ref. 8). These observations suggested that combined exposure to antigen and EBV might result in the establishment of cell lines enriched for antigen-sensitive or antibody-forming cells.
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BAUMAL, R., BLOOM, B. & SCHARFF, M. Induction of Long Term Lymphocyte Lines from Delayed Hypersensitive Human Donors using Specific Antigen plus Epstein–Barr Virus. Nature New Biology 230, 20–21 (1971). https://doi.org/10.1038/newbio230020a0
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DOI: https://doi.org/10.1038/newbio230020a0