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Buoyant Density and Satellite Composition of DNA of Mouse Heterochromatin

Nature New Biology volume 229, pages 175–176 (1971)Cite this article

Abstract

Ten per cent of mouse DNA occurs as a satellite band with a buoyant density lighter than that of the main band1. This satellite contains highly repetitious DNA2,3. It has been shown that the amount of satellite is markedly increased in DNA isolated from the heterochromatin fraction of mouse nuclei4. Furthermore, in situ hybridization studies have shown that satellite DNA is localized to the pericentromeric heterochromatin of all the mouse chromosomes except the Y5,6. These observations demonstrate an intimate association between mouse satellite DNA and heterochromatin and they raise the question: is all the DNA from mouse heterochromatin composed of satellite DNA or is a significant portion composed of non-satellite DNA?

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Authors and Affiliations

  1. Department of Medical Genetics, City of Hope Medical Center, Duarte, California, 91010

    EMILIO MATTOCCIA & DAVID E. COMINGS

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  1. EMILIO MATTOCCIA
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  2. DAVID E. COMINGS
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MATTOCCIA, E., COMINGS, D. Buoyant Density and Satellite Composition of DNA of Mouse Heterochromatin. Nature New Biology 229, 175–176 (1971). https://doi.org/10.1038/newbio229175a0

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