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Formation of Hybrid Cells and Heterokaryons by Fusion of Enucleated and Nucleated Cells

Nature New Biology volume 229, pages 123–125 (1971)Cite this article

Abstract

OBSERVATIONS on the synchronous behaviour of nuclei in naturally occurring binucleate and multinucleate cells have indicated that certain nuclear events are regulated by cytoplasmic factors1,2. And the importance of cytoplasmic factors in regulating gene expression has been demonstrated in hybrid cells and heterokaryons formed by fusion of cells through the use of inactivated Sendai virus3,4. We wish to describe a modification of virus-induced cell fusion, whereby hybrids and heterokaryons can be created by fusing nucleated cells of one species with enucleated cells from another. Using this technique the relative contribution of nucleus and cytoplasm in controlling the expression of specific functions can be better assessed.

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Authors and Affiliations

  1. Department of Virology, Royal Postgraduate Medical School, Du Cane Road, London, W12

    G. POSTE & P. REEVE

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  1. G. POSTE
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  2. P. REEVE
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POSTE, G., REEVE, P. Formation of Hybrid Cells and Heterokaryons by Fusion of Enucleated and Nucleated Cells. Nature New Biology 229, 123–125 (1971). https://doi.org/10.1038/newbio229123a0

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