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Isolabelling and Chromosome Strandedness

Abstract

IF cells of eukaryotes are pulse-labelled with tritiated thymidine and then allowed to pass through mitosis (M1) and complete a second round of DNA replication (S2) without label, at the next mitosis (M2) the label is seen to segregate semi-conservatively; that is, with the exception of sister chromatid exchanges, all the label will pass to one chromatid1–4.

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COMINGS, D. Isolabelling and Chromosome Strandedness. Nature New Biology 229, 24–25 (1971). https://doi.org/10.1038/newbio229024a0

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