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How evolving enzymes can beat the heat and avoid defeat

Nature Chemical Biology volume 5, pages 538–539 (2009)Cite this article

Random mutations usually destabilize protein tertiary structure. Overexpression of the chaperonin GroEL/GroES protects evolving proteins from the destabilizing effects of adaptive mutations and improves the quantity and quality of enzyme variants with modified function.

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Figure 1: Chaperonin overexpression accelerates adaptive protein evolution.

References

  1. Tokuriki, N. & Tawfik, D.S. Nature 459, 668–673 (2009).

    Article  CAS  PubMed  Google Scholar 

  2. Bloom, J.D., Labthavikul, S.T., Otey, C.R. & Arnold, F.H. Proc. Natl. Acad. Sci. USA 103, 5869–5874 (2006).

    Article  CAS  PubMed  Google Scholar 

  3. Bershtein, S., Goldin, K. & Tawfik, D.S. J. Mol. Biol. 379, 1029–1044 (2008).

    Article  CAS  PubMed  Google Scholar 

  4. Bloom, J.D. et al. BMC Biol. 5, 29 (2007).

    Article  PubMed  PubMed Central  Google Scholar 

  5. Rutherford, S.L. Nat. Rev. Genet. 4, 263–274 (2003).

    Article  CAS  PubMed  Google Scholar 

  6. Tomala, K. & Korona, R. Biol. Direct 3, 5 (2008).

    Article  PubMed  PubMed Central  Google Scholar 

  7. Ignatova, Z. & Gierasch, L.M. Proc. Natl. Acad. Sci. USA 103, 13357–13361 (2006).

    Article  CAS  PubMed  Google Scholar 

  8. Panavas, T., Sanders, C. & Butt, T.R. Methods Mol. Biol. 497, 303–317 (2009).

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Ichiro Matsumura and Andrei A. Ivanov are in the Department of Biochemistry, Center for Fundamental and Applied Molecular Evolution, Emory University School of Medicine, Atlanta, Georgia, USA.,

    Ichiro Matsumura & Andrei A Ivanov

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  1. Ichiro Matsumura
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  2. Andrei A Ivanov
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Correspondence to Ichiro Matsumura.

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Matsumura, I., Ivanov, A. How evolving enzymes can beat the heat and avoid defeat. Nat Chem Biol 5, 538–539 (2009). https://doi.org/10.1038/nchembio0809-538

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