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Synthetic GPI array to study antitoxic malaria response

Nature Chemical Biology volume 4, pages 238–240 (2008)Cite this article

Abstract

Parasite glycosylphosphatidylinositol (GPI) is an important toxin in malaria disease, and people living in malaria-endemic regions often produce high levels of anti-GPI antibodies. The natural anti-GPI antibody response needs to be understood to aid the design of an efficient carbohydrate-based antitoxin vaccine. We present a versatile approach based on a synthetic GPI glycan array to correlate anti-GPI antibody levels and protection from severe malaria.

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Figure 1: Synthetic GPI glycans for microarray construction.
Figure 2: ELISA analysis of serum samples using synthetic GPI glycan microarray.

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Acknowledgements

We thank the Swiss National Science Foundation (SNF grant 205321-107651 to P.H.S. and 310000-116337/1 to G.P.), the ETH Zürich and the Fondation Bay for generous support of this research. A European Molecular Biology Organization long-term fellowship (to F.K.) is gratefully acknowledged. We thank J. Sobek for assistance in array printing.

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Authors and Affiliations

  1. Laboratory for Organic Chemistry, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Str. 10, Zurich, 8093, Switzerland

    Faustin Kamena, Xinyu Liu, Yong-Uk Kwon & Peter H Seeberger

  2. Swiss Tropical Institute, Socinstr. 57, Basel, 4002, Switzerland

    Marco Tamborrini & Gerd Pluschke

  3. Nuffield Department of Clinical Medicine, Center for Clinical Vaccinology and Tropical Medicine, Oxford University, Churchill Hospital, Old Road, Oxford, OX3 7LJ, UK

    Fiona Thompson

Authors
  1. Faustin Kamena
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  2. Marco Tamborrini
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  3. Xinyu Liu
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  4. Yong-Uk Kwon
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  5. Fiona Thompson
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  6. Gerd Pluschke
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  7. Peter H Seeberger
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Contributions

F.K., G.P. and P.H.S. designed the research. F.K., M.T. and X.L. performed the research. X.L., Y.-U.K. and F.T. contributed new reagents and analytic tools. F.K., M.T., G.P. and P.H.S. analyzed the data. F.K., M.T., X.L., G.P. and P.H.S. wrote the paper with contributions from the other authors.

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Correspondence to Peter H Seeberger.

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Kamena, F., Tamborrini, M., Liu, X. et al. Synthetic GPI array to study antitoxic malaria response. Nat Chem Biol 4, 238–240 (2008). https://doi.org/10.1038/nchembio.75

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