The extent to which ligand-free GPCRs exist in quasi-stable 'precoupled' complexes with G proteins in vivo is uncertain. New research, using fluorescence recovery after photobleaching (FRAP), reveals a structural requirement for and functional consequences of muscarinic acetylcholine receptor–Gq protein preassembly.
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Challiss, R., Wess, J. GPCR–G protein preassembly?. Nat Chem Biol 7, 657–658 (2011). https://doi.org/10.1038/nchembio.665
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DOI: https://doi.org/10.1038/nchembio.665