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Post-translational modifications

A shift for the O-GlcNAc paradigm

Many nuclear and cytoplasmic proteins are post-translationally modified by both O-GlcNAc and phosphate, but determining whether a single copy of a protein bears multiple modifications remains a challenge. A new analytic approach reveals a surprising correlation between phosphorylation and the O-GlcNAc modification.

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Figure 1: Mapping multiple post-translational modifications.

References

  1. Torres, C.R. & Hart, G.W. J. Biol. Chem. 259, 3308–3317 (1984).

    CAS  Google Scholar 

  2. Hart, G.W., Housley, M.P. & Slawson, C. Nature 446, 1017–1022 (2007).

    Article  CAS  Google Scholar 

  3. Shafi, R. et al. Proc. Natl. Acad. Sci. USA 97, 5735–5739 (2000).

    Article  CAS  Google Scholar 

  4. McClain, D.A. et al. Proc. Natl. Acad. Sci. USA 99, 10695–10699 (2002).

    Article  CAS  Google Scholar 

  5. Hanover, J.A. et al. Proc. Natl. Acad. Sci. USA 102, 11266–11271 (2005).

    Article  CAS  Google Scholar 

  6. Fujiki, R. et al. Nature 459, 455–459 (2009).

    Article  CAS  Google Scholar 

  7. Rexach, J. E. et al. Nat. Chem. Biol. 6, 645–651 (2010).

    Article  CAS  Google Scholar 

  8. Hart, G.W. et al. Adv. Exp. Med. Biol. 376, 115–123 (1995).

    Article  CAS  Google Scholar 

  9. Wells, L., Kreppel, L.K., Comer, F.I., Wadzinski, B.E. & Hart, G.W. J. Biol. Chem. 279, 38466–38470 (2004).

    Article  CAS  Google Scholar 

  10. Mikesh, L.M. et al. Biochim. Biophys. Acta 1764, 1811–1822 (2006).

    Article  CAS  Google Scholar 

  11. Tao, J. et al. Proc. Natl. Acad. Sci. USA 106, 4882–4887 (2009).

    Article  CAS  Google Scholar 

Download references

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Correspondence to Jennifer J Kohler.

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Kohler, J. A shift for the O-GlcNAc paradigm. Nat Chem Biol 6, 634–635 (2010). https://doi.org/10.1038/nchembio.429

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  • DOI: https://doi.org/10.1038/nchembio.429

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