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A scaffold protein that chaperones a cysteine-sulfenic acid in H2O2 signaling

Nature Chemical Biology volume 13, pages 909–915 (2017)Cite this article

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Abstract

In Saccharomyces cerevisiae, Yap1 regulates an H2O2-inducible transcriptional response that controls cellular H2O2 homeostasis. H2O2 activates Yap1 by oxidation through the intermediary of the thiol peroxidase Orp1. Upon reacting with H2O2, Orp1 catalytic cysteine oxidizes to a sulfenic acid, which then engages into either an intermolecular disulfide with Yap1, leading to Yap1 activation, or an intramolecular disulfide that commits the enzyme into its peroxidatic cycle. How the first of these two competing reactions, which is kinetically unfavorable, occurs was previously unknown. We show that the Yap1-binding protein Ybp1 brings together Orp1 and Yap1 into a ternary complex that selectively activates condensation of the Orp1 sulfenylated cysteine with one of the six Yap1 cysteines while inhibiting Orp1 intramolecular disulfide formation. We propose that Ybp1 operates as a scaffold protein and as a sulfenic acid chaperone to provide specificity in the transfer of oxidizing equivalents by a reactive sulfenic acid species.

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Figure 1: Schematics of the dual function of Orp1 as the H2O2 receptor of the Yap1 regulator and as a peroxidase.
Figure 2: Ybp1 brings together Yap1 and Orp1 into a ternary complex.
Figure 3: Ybp1 is required for Orp1–S–S–Yap1 intermediate formation.
Figure 4: Ybp1 activates Orp1–S–S–Yap1 formation.
Figure 5: The Ybp1·Yap1 complex inhibits Orp1 intramolecular disulfide formation.
Figure 6: Lack of Orp1 resolving C82 does not make Ybp1 dispensable for Yap1 oxidation.

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Acknowledgements

We gratefully acknowledge G. Branlant for his essential input to initiate the project. We also thank A. Gruez for assistance with protein stability optimization; S. Boschi-Muller and F. Talfournier for fruitful discussions and help with quench flow experiments; J.M. Alberto for support with the use of the NGERE U954-INSERM chromatographic facility; and J. Charbonnel and G. Palais for excellent technical support. Microcalorimetry and mass spectrometry were performed respectively at the SCBIM (Fédération de Recherche 3209 BMCT) and SCMS platforms of Université de Lorraine. This work was supported by grants from the Ligue contre le Cancer to S.R.-C., and from ANR ERRed, InCA PLBIO INCA_5869 to M.B.T. A.B. was supported by a PhD fellowship from the French research minister.

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  1. Gemma Belli

    Present address: Department of Basic Medical Sciences, IRB Lleida, University of Lleida, Lleida, Spain

Authors and Affiliations

  1. UMR 7365 CNRS–Université de Lorraine IMoPA, Biopôle, Campus Biologie-Santé, Vandœuvre-lès-Nancy, France

    Antoine Bersweiler, Hortense Mazon, Alexandre Kriznik & Sophie Rahuel-Clermont

  2. Institute for Integrative Biology of the Cell (I2BC), CNRS, CEA–Saclay, Université Paris–Saclay, iBiTecS/SBIGEM, Laboratoire Stress Oxydant et Cancer, Gif-sur-Yvette, France

    Benoît D'Autréaux, Gemma Belli, Agnès Delaunay-Moisan & Michel B Toledano

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  1. Antoine Bersweiler
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Contributions

A.B., B.d'A., M.B.T., and S.R.-C. designed the experiments and analyzed the data. A.B., A.K., and B.d'A. produced and purified the proteins, A.B. and B.d'A. performed in vitro reconstitution assays, B.d'A. on gels kinetics and A.B. all other kinetics; H.M. and A.B. performed chromatography and mass spectrometry analyses; A.B. and A.K. performed protein interaction experiments. G.B., B.d'A., and A.D.-M. carried out in vivo experiments. S.R.-C., B.d'A., and M.B.T. wrote the manuscript.

Corresponding authors

Correspondence to Michel B Toledano or Sophie Rahuel-Clermont.

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Bersweiler, A., D'Autréaux, B., Mazon, H. et al. A scaffold protein that chaperones a cysteine-sulfenic acid in H2O2 signaling. Nat Chem Biol 13, 909–915 (2017). https://doi.org/10.1038/nchembio.2412

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