Brief Communication | Published:

The Taf14 YEATS domain is a reader of histone crotonylation

Nature Chemical Biology volume 12, pages 396398 (2016) | Download Citation

Abstract

The discovery of new histone modifications is unfolding at startling rates; however, the identification of effectors capable of interpreting these modifications has lagged behind. Here we report the YEATS domain as an effective reader of histone lysine crotonylation, an epigenetic signature associated with active transcription. We show that the Taf14 YEATS domain engages crotonyllysine via a unique π–π–π-stacking mechanism and that other YEATS domains have crotonyllysine-binding activity.

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Acknowledgements

We thank L. Aponte-Collazo for help with experiments and M. Bedford, K. Chua, D. Stillman, and J. Tyler for kindly providing some original DNA constructs of YEATS and bromodomains, as well as the yeast strains and extracts. This work was supported by NIH grants R01 GM106416 and GM100907 to T.G.K. and GM110058 to B.D.S. and by CPRIT RP160237 to X.S. F.H.A. is supported by the NIH grant T32AA007464, E.K.S. is supported by the NIH grant K12-GM000678, S.A.S. is supported by a UNC Lineberger Cancer Center Postdoctoral Fellowship Award, and A.G. is supported by the CPRIT Research Training grant RP140106.

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Author notes

    • Forest H Andrews
    •  & Stephen A Shinsky

    These authors contributed equally to this work.

Affiliations

  1. Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, USA.

    • Forest H Andrews
    •  & Tatiana G Kutateladze
  2. Department of Biochemistry & Biophysics, the University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.

    • Stephen A Shinsky
    • , Erin K Shanle
    • , Joseph B Bridgers
    • , Ian K Tsun
    • , Krzysztof Krajewski
    •  & Brian D Strahl
  3. Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, Texas, USA.

    • Anneliese Gest
    •  & Xiaobing Shi

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Contributions

F.H.A., S.A.S., E.K.S., J.B.B., A.G., I.K.T., and K.K. performed experiments and, together with X.S., B.D.S., and T.G.K., analyzed the data. F.H.A., S.A.S., B.D.S., and T.G.K. wrote the manuscript with input from all authors.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Brian D Strahl or Tatiana G Kutateladze.

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DOI

https://doi.org/10.1038/nchembio.2065

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