Abstract
The discovery of new histone modifications is unfolding at startling rates; however, the identification of effectors capable of interpreting these modifications has lagged behind. Here we report the YEATS domain as an effective reader of histone lysine crotonylation, an epigenetic signature associated with active transcription. We show that the Taf14 YEATS domain engages crotonyllysine via a unique π–π–π-stacking mechanism and that other YEATS domains have crotonyllysine-binding activity.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Tan, M. et al. Cell 146, 1016–1028 (2011).
Sabari, B.R. et al. Mol. Cell 58, 203–215 (2015).
Lin, H., Su, X. & He, B. ACS Chem. Biol. 7, 947–960 (2012).
Bao, X. et al. eLife 3, e02999 (2014).
Musselman, C.A., Lalonde, M.E., Côté, J. & Kutateladze, T.G. Nat. Struct. Mol. Biol. 19, 1218–1227 (2012).
Rothbart, S.B. & Strahl, B.D. Biochim. Biophys. Acta 1839, 627–643 (2014).
Flynn, E.M. et al. Structure 23, 1801–1814 (2015).
Filippakopoulos, P. et al. Cell 149, 214–231 (2012).
Schulze, J.M., Wang, A.Y. & Kobor, M.S. Biochem. Cell Biol. 87, 65–75 (2009).
Li, Y. et al. Cell 159, 558–571 (2014).
Shanle, E.K. et al. Genes Dev. 29, 1795–1800 (2015).
Kabani, M., Michot, K., Boschiero, C. & Werner, M. Biochem. Biophys. Res. Commun. 332, 398–403 (2005).
Shen, X. Methods Enzymol. 377, 401–412 (2004).
Cairns, B.R., Henry, N.L. & Kornberg, R.D. Mol. Cell. Biol. 16, 3308–3316 (1996).
John, S. et al. Genes Dev. 14, 1196–1208 (2000).
Andrews, F.H., Shanle, E.K., Strahl, B.D. & Kutateladze, T.G. Transcription 7, 14–20 (2016).
McCoy, A.J. et al. J. Appl. Crystallogr. 40, 658–674 (2007).
Adams, P.D. et al. Acta Crystallogr. D Biol. Crystallogr. 66, 213–221 (2010).
Emsley, P., Lohkamp, B., Scott, W.G. & Cowtan, K. Acta Crystallogr. D Biol. Crystallogr. 66, 486–501 (2010).
Ali, M. et al. Proc. Natl. Acad. Sci. USA 110, 11296–11301 (2013).
Rothbart, S.B. et al. Nat. Struct. Mol. Biol. 19, 1155–1160 (2012).
Shanle, E.K. et al. Genes Dev. 29, 1795–1800 (2015).
Keogh, M.C. et al. Genes Dev. 20, 660–665 (2006).
Acknowledgements
We thank L. Aponte-Collazo for help with experiments and M. Bedford, K. Chua, D. Stillman, and J. Tyler for kindly providing some original DNA constructs of YEATS and bromodomains, as well as the yeast strains and extracts. This work was supported by NIH grants R01 GM106416 and GM100907 to T.G.K. and GM110058 to B.D.S. and by CPRIT RP160237 to X.S. F.H.A. is supported by the NIH grant T32AA007464, E.K.S. is supported by the NIH grant K12-GM000678, S.A.S. is supported by a UNC Lineberger Cancer Center Postdoctoral Fellowship Award, and A.G. is supported by the CPRIT Research Training grant RP140106.
Author information
Authors and Affiliations
Contributions
F.H.A., S.A.S., E.K.S., J.B.B., A.G., I.K.T., and K.K. performed experiments and, together with X.S., B.D.S., and T.G.K., analyzed the data. F.H.A., S.A.S., B.D.S., and T.G.K. wrote the manuscript with input from all authors.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Text and Figures
Supplementary Results, Supplementary Figures 1–7 and Supplementary Tables 1 and 2. (PDF 13029 kb)
Rights and permissions
About this article
Cite this article
Andrews, F., Shinsky, S., Shanle, E. et al. The Taf14 YEATS domain is a reader of histone crotonylation. Nat Chem Biol 12, 396–398 (2016). https://doi.org/10.1038/nchembio.2065
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/nchembio.2065
This article is cited by
-
The mechanisms, regulations, and functions of histone lysine crotonylation
Cell Death Discovery (2024)
-
Chromatin balances cell redox and energy homeostasis
Epigenetics & Chromatin (2023)
-
Epigenetic meets metabolism: novel vulnerabilities to fight cancer
Cell Communication and Signaling (2023)
-
Emerging posttranslational modifications and their roles in DNA damage response
Genome Instability & Disease (2023)
-
Oncometabolites drive tumorigenesis by enhancing protein acylation: from chromosomal remodelling to nonhistone modification
Journal of Experimental & Clinical Cancer Research (2022)