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Synthetic biology

Two-for-one designer labels

Nature Chemistry volume 6, pages 379–381 (2014)Cite this article

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Labelling of proteins with pairs of fluorophores enables their conformations to be studied; however, complete incorporation of labels in multiple, pre-defined locations is very difficult. Now, a combination of double unnatural amino acid mutagenesis and selective chemical modification offers a general method to achieve this.

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Figure 1: Efficient double labelling of a protein for FRET studies is made possible by orthogonal machinery for unnatural amino acid incorporation and orthogonal chemical reactions.

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Authors and Affiliations

  1. E. James Petersson and John B. Warner are at the Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, Pennsylvania 19104-6323, USA,

    E. James Petersson & John B. Warner

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  1. E. James Petersson
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  2. John B. Warner
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Correspondence to E. James Petersson.

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Petersson, E., Warner, J. Two-for-one designer labels. Nature Chem 6, 379–381 (2014). https://doi.org/10.1038/nchem.1935

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