The most complex non-DNA synthetic molecular knot so far has been made in a single step by combining a number of reversible chemical interactions, including metal-directed self-assembly, anion templation and imine bond formation.
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Hardie, M. One-pot pentaknot. Nature Chem 4, 7–8 (2012). https://doi.org/10.1038/nchem.1224
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DOI: https://doi.org/10.1038/nchem.1224