CRISPR/Cas9-edited NSG mice as PDX models of human leukemia to address the role of niche-derived SPARC

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Acknowledgements

We thank Dr Henner Farin and Dr Michael Milsom for helpful discussions, Maresa Weitmann for technical help and Dr Michaela Socher, Dr Boris Brill and all members of the DKFZ and GSH Laboratory Animal Core Facility for excellent animal welfare and husbandry. We thank Dr Sebastian Wagner and Dr Khalil Abou Elaradat from the Frankfurt DKTK sequencing facility for the MiSeq runs and Dr Stefan Stein for the THP-1 line. We thank H Altmann and C Röllig from the SAL biobank (Dresden) and Dr Vick Binje from the Helmholtz Center (Munich) for providing samples. HM is supported by the European Research Council (ERC Grant No. 639795) and the German José Carreras Leukemia Foundation (Award No. DJCLS A 14/01).

Author contributions

IT-G, AN, EC, DS, EB, AC and HM performed experiments. AM performed the analysis of NGS data. UK and FVdH isolated the NSG zygotes and performed the cytoplasmic microinjections. IJ, JPB and UP contributed reagents and discussed results. HM designed and supervised the study. HM and ITG wrote the manuscript.

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Correspondence to H Medyouf.

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The authors declare no conflict of interest.

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Supplementary Information accompanies this paper on the Leukemia website

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Tirado-Gonzalez, I., Czlonka, E., Nevmerzhitskaya, A. et al. CRISPR/Cas9-edited NSG mice as PDX models of human leukemia to address the role of niche-derived SPARC. Leukemia 32, 1048–1051 (2018) doi:10.1038/leu.2017.346

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