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Effect of 17-α hydroxyprogesterone caproate on the production of tumor necrosis factor-α and the expression of cyclooxygenase-2 in lipopolysaccharide-treated gravid human myometrial explants

Journal of Perinatology volume 30, pages 584–589 (2010)Cite this article

Subjects

Abstract

Objective:

To determine whether 17-α hydroxyprogesterone (17-OHPC) alters tumor necrosis factor-α (TNF-α) production and the expression of cyclooxygenase type 2 (COX-2) in myometrium exposed to lipopolysaccharide (LPS).

Study Design:

Lower segment myometrial biopsies were obtained from non-laboring patients at term. Tissues were cultured in serum-free media with 17-OHPC (1 μM) and LPS (1 μg/ml), either alone or in combination. At 24 h, the production of tumor necrosis factor-α (TNF-α) and the expression of COX-2 was determined using enzyme linked immunosorbent assay and real-time (RT-PCR). Statistical analysis was performed using non-parametric testing. A P-value of <0.05 was considered significant.

Result:

17-OHPC had no effect on TNF-α production and COX-2 expression when compared with untreated myometrial explants (P=0.61 and P=0.95). LPS induced production of TNF-α (P=0.03) and expression of COX-2 (P=0.02). Treatment with 17-OHPC did not block LPS-induced TNF-α production (P=0.37) or COX-2 expression (P=0.12).

Conclusion:

In this pilot study, 17-OHPC did not affect the production of TNF-α or COX-2 expression in human myometrium.

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Acknowledgements

We thank Drs Frank Stanczyk and Durlin Hickok and Adeza Biomedical Corporation for providing the 17-OHPC used in our experiment. We also acknowledge grant funding from Health Research Associates.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Los Angeles County–University of Southern California Medical Center, Los Angeles, CA, USA

    S Patel, T M Goodwin, M Blitz, J C Felix & R H Lee

  2. Department of Pediatrics, Los Angeles County–University of Southern California Medical Center, Los Angeles, CA, USA

    A Li & P Minoo

  3. Department of Obstetrics and Gynecology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA

    M Brower

  4. Department of Pathology, Los Angeles County–University of Southern California Medical Center, Los Angeles, CA, USA

    J C Felix

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  1. S Patel
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Correspondence to R H Lee.

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Patel, S., Li, A., Goodwin, T. et al. Effect of 17-α hydroxyprogesterone caproate on the production of tumor necrosis factor-α and the expression of cyclooxygenase-2 in lipopolysaccharide-treated gravid human myometrial explants. J Perinatol 30, 584–589 (2010). https://doi.org/10.1038/jp.2010.23

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