Summary
An ability of the fibroblasts to repair ultraviolet (UV)-induced damage of DNA was investigated in patients with systemic lupus erythematosus (SLE). Unscheduled DNA synthesis (UDS) and survival rate in SLE fibroblasts were not reduced in comparison with those in normal fibroblasts, as Cleaver had reported previously (1970). In addition, sera from SLE patients had no effects on UDS. In the fibroblast colony studies, some clones exhibited decreased UDS, though mean UDS of all colonies was approximately same among different individuals. This intra-individual clonal variation in UDS occurred in both SLE patients and controls. These results indicate that the capacity of DNA repair of the fibroblasts from SLE patients is at the same level as those from normal individuals. In SLE patients, however, such clone(s) of cells with decreased level of DNA repair may present the damaged DNA induced by UV light as antigen, which will form the immune complexes under an abnormal immune responsiveness, leading to the development of photosensitivity.
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Ohta, A., Morito, F. & Yamaguchi, M. Study of DNA repair of the fibroblasts from patients with systemic lupus erythematosus. Jap J Human Genet 31, 357–364 (1986). https://doi.org/10.1007/BF01907936
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DOI: https://doi.org/10.1007/BF01907936