Summary
A simple R-banding technique was presented. Peripheral leukocytes were incorporated with 5′-bromodeoxy uridine for 6 to 8 hr prior to havesting. R-banded metaphases were obtained by Giemsa staining following 33258 Hoechst staining for 15 min and heating at 60°C for 80 min in 2×SSC with exposure to ultraviolet. This method has many advantages; 1) the possibility of obtaining a permanent R-banding preparation immediately after harvest, 2) feasible demonstration of the latereplicating X chromosome, and 3) no requirement of a fluorescence microscope.
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Ieshima, A., Yorita, T. & Takeshita, K. A simple R-banding technique by BrdU-Hoechst treatment and Giemsa staining following heating and ultraviolet exposure. Jap J Human Genet 29, 133–138 (1984). https://doi.org/10.1007/BF01873534
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DOI: https://doi.org/10.1007/BF01873534
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