Summary
In vivo genome mutagenicity of colchicine in the primary oocytes of the Chinese hamster was analyzed at a selected dose that did not completely arrest the formation of the first meiotic spindle, but exhibited a remarkable induction of nondisjunction, very possibly as the result of the inhibition of tubulin polymerization.
A single dose of 3 μg/g b.w. colchicine was intraperitoneally administered to females with a normal estrous cycle at the onset of the formation of the first meiotic spindle. Chromosome analysis of a total of 2,124 secondary oocytes revealed that the incidence of aneuploids increased significantly (p<0.001) in the experimental group (25.9%, 99 out of 382) as compared with the control group (2.0%, 35 out of 1,742). The minimum genome mutagenicity of colchicine caused nondisjunction in one or two bivalents and eventually induced a large number of aneuploid secondary oocytes within haploid range.
The condition of the spindle in oocytes aged by pre- and postovulatory overripeness, both of which have been known to induce meiotic chromosomal nondisjunction, were discussed in relation to tubulin polymerization.
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Sugawara, S., Mikamo, K. Colchicine-induced abnormal meiotic chromosomal segregation in primary oocytes of the Chinese hamster. Jap J Human Genet 25, 235–240 (1980). https://doi.org/10.1007/BF01997701
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DOI: https://doi.org/10.1007/BF01997701
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