Abstract
Benanomicins were found as antifungal antibiotics from the culture of an actinomycete with potent antifungal activities in vitro and in vivo. We aimed to generate derivatives superior to benanomicin A by biotransformation using Escherichia coli constructed with bacterial P450 expression system. We found transformation of benanomicin A into two derivatives, 10-hydroxybenanomicin A and 11-O-demethylbenanomicin A by one of the P450-expressed strains which harbored a plasmid carrying a CYP105C1-homologous gene. Unexpectedly, the biotransformed compounds showed weak antifungal activities in vitro compared with those of benanomicin A.
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Kumagai, H., Umekita, M., Sawa, R. et al. Generation of New Benanomicin Analogues by Biotransformation Using Escherichia coli Expressing Actinomycete Cytochrome P450. J Antibiot 61, 394–399 (2008). https://doi.org/10.1038/ja.2008.55
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DOI: https://doi.org/10.1038/ja.2008.55
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