Abstract
A novel cyclic peptide antibiotic, pargamicin A was isolated from the culture broth of an actinomycete strain. The producing organism, designated ML1-hF4, was identified as a member of the genus Amycolatopsis. Pargamicin A was identified as a novel cyclic hexapeptide antibiotic containing piperazic acid by various spectroscopic analyses. Pargamicin A showed potent antibacterial activity against Staphylococcus aureus strains including MRSA and Enterococcus faecalis/faecium strains including VRE.
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Tenover FC, Weigel LM, Appelbaum PC, McDougal LK, Chaitram J, McAllister S, Clark N, Killgore G, O'Hara CM, Jevitt L, Patel JB, Bozdogan B . Vancomycin-resistant Staphylococcus aureus isolate from a patient in Pennsylvania. Antimicrob Agents Chemother 48: 275–280 ( 2004)
Rybak MJ, Hershberger E, Moldovan T, Grucz RG . In vitro activities of daptomycin, vancomycin, linezolid, and quinupristin-dalfopristin against Staphylococci and Enterococci, including vancomycin-intermediate and -resistant strains. Antimicrob Agents Chemother 44: 1062–1066 ( 2000)
Shiring EB, Gottlieb D . Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313–340 ( 1966)
Waksman SA . Classification, identification and descriptions of genera and species. In: The Actinomycetes, Vol. II. Williams & Wilkins, Baltimore, pp. 327–334 ( 1961)
Staneck JL, Roberts GD . Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28: 226–231 ( 1974)
Lechevalier MP, Lechevalier HA . The chemotaxonomy of actinomycetes. In: Dietz AD, et al. (ed). Actinomycete Taxonomy. Society for Industrial Microbiology, Virginia, pp. 227–291 ( 1980)
Collins MD, Pirouz T, Goodfellow M, Minnikin DE . Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221–230 ( 1977)
Minnikin DE, Patel PV, Alshamaony L, Goodfellow M . Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27: 104–117 ( 1977)
Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M . Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatography 188: 221–233 ( 1980)
Hamada M, Kinoshita N, Hattori S, Yoshida A, Okami Y, Higashide K, Sakata N, Hori M . Streptomyces kasugaensis sp. nov.: A new species of genus Streptomyces. Actinomycetol 9: 27–36 ( 1995)
Kinoshita N, Igarashi M, Ikeno S, Hori M, Hamada M . Saccharothrix tangerinus sp. nov., the producer of the new antibiotic formamicin: taxonomic studies. Actinomycetol 13: 20–31 ( 1999)
Japan Society of Chemotherapy. Method of MIC determination. Chemother 29: 77–79 ( 1981) ( in Japanese)
Brosius J, Palmer ML, Kennedy PJ, Noller HF . Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75: 4801–4805 ( 1978)
Lechevalier MP, Prauser H, Labeda DP, Ruan JS . Two new genera of Nocardioform Actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36: 29–37 ( 1986)
Pettibone DJ, Clineschmidt BV, Anderson PS, Freidinger RM, Lundell GF, Koupal LR, Schwartz CD, Williamson JM, Goetz MA, Hensens OD, et al. A structurally unique, potent, and selective oxytocin antagonist derived from Streptomyces silvensis. Endocrinology 125: 217–222 ( 1989)
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Igarashi, M., Sawa, R., Kinoshita, N. et al. Pargamicin A, a Novel Cyclic Peptide Antibiotic from Amycolatopsis sp.. J Antibiot 61, 387–393 (2008). https://doi.org/10.1038/ja.2008.54
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DOI: https://doi.org/10.1038/ja.2008.54
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