Abstract
LC/MS/MS under ion trap conditions was used to analyze microcystins produced by cyanobacteria. Tandem mass spectrometry using MS2 was quite effective since ions arising from cleavage at a peptide bond provide useful sequence information. The fragmentation was confirmed by a shifting technique using structurally-related microcystins and the resulting fragmentation pattern was different from those determined by triple stage MS/MS and four sector MS/MS. Analysis of a mixture of microcystins in a bloom sample was successfully performed and two new microcystins were identified by LC/MS/MS under ion trap conditions. Thus, LC/MS/MS under ion trap conditions is effective for the structural characterization of microcystins.
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Mayumi, T., Kato, H., Imanishi, S. et al. Structural Characterization of Microcystins by LC/MS/MS under Ion Trap Conditions. J Antibiot 59, 710–719 (2006). https://doi.org/10.1038/ja.2006.95
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DOI: https://doi.org/10.1038/ja.2006.95
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