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Microbial contributions to the persistence of coral reefs

The ISME Journal volume 11, pages 21672174 (2017) | Download Citation


On contemplating the adaptive capacity of reef organisms to a rapidly changing environment, the microbiome offers significant and greatly unrecognised potential. Microbial symbionts contribute to the physiology, development, immunity and behaviour of their hosts, and can respond very rapidly to changing environmental conditions, providing a powerful mechanism for acclimatisation and also possibly rapid evolution of coral reef holobionts. Environmentally acquired fluctuations in the microbiome can have significant functional consequences for the holobiont phenotype upon which selection can act. Environmentally induced changes in microbial abundance may be analogous to host gene duplication, symbiont switching / shuffling as a result of environmental change can either remove or introduce raw genetic material into the holobiont; and horizontal gene transfer can facilitate rapid evolution within microbial strains. Vertical transmission of symbionts is a key feature of many reef holobionts and this would enable environmentally acquired microbial traits to be faithfully passed to future generations, ultimately facilitating microbiome-mediated transgenerational acclimatisation (MMTA) and potentially even adaptation of reef species in a rapidly changing climate. In this commentary, we highlight the capacity and mechanisms for MMTA in reef species, propose a modified Price equation as a framework for assessing MMTA and recommend future areas of research to better understand how microorganisms contribute to the transgenerational acclimatisation of reef organisms, which is essential if we are to reliably predict the consequences of global change for reef ecosystems.

Coral reefs under climate change

Corals are fundamental ecosystem engineers, responsible for constructing large intricate reefs that support diverse and abundant marine life. Central to a healthy coral animal is a dynamic relationship with diverse eukaryotic, prokaryotic and viral symbionts, collectively termed the coral holobiont (Margulis, 1991). Although coral reefs have only a relatively small spatial extent, their topography and unique primary production support ~25% of all described marine species (Knowlton and Jackson, 2001). However, coral reefs are also critically threatened habitats currently experiencing significant global declines attributed to localised impacts such as poor water quality including elevated nutrients, sediments and pollutants, and global climate shifts driving elevated sea surface temperatures and ocean acidification ((De’ath et al., 2012) and references therein). Scleractinian corals live so close to their upper thermal limit that even slight temperature changes can result in large-scale bleaching events (Hoegh-Guldberg et al., 2007; Hughes et al., 2017). Whether or not coral reefs can adapt sensu lato to these cumulative environmental pressures is a topic of current debate. The sexual generation time for most reef species (~4–8 years in corals) is generally considered too long for genetic adaptation to keep pace with current rates of climate change (Reusch, 2014). However, recent evidence of increased bleaching tolerance or inheritance of heat tolerance indicates that at least some reef species have the ability to rapidly (days to years) respond to changing climatic conditions ((Palumbi et al., 2014); Supplementary Table 1).

Adaptation sensu lato in the coral holobiont

Broad-sense adaptation to environmental change may occur through both genetic (that is, evolutionary adaptation) and non-genetic (that is, acclimatisation) mechanisms (Box 1). Genetic adaptation is often described as hard inheritance because it involves a change in the genotype pool from one generation to the next through natural selection, a process that occurs at the population level. The mechanistic basis is a change in the DNA or RNA sequence, which then changes gene function and/or gene expression, ultimately resulting in altered mean phenotypes. By definition, the adapted population then displays increased Darwinian fitness. In host organisms such as long-lived corals, this is supposed to be a slow process, notwithstanding the potential for within-colony (epi)genetic changes (van Oppen et al., 2015). Somatic mutations as one possible route to ‘within-coral colony’ adaptation has also recently been proposed and merits further investigation to assess its ubiquity in coral reef systems (Schweinsberg et al., 2015).

Box 1: Defining evolutionary concepts:

Evolutionary adaptation=adaptation sensu stricto describes a population-level change in the mean phenotype from one generation to the next due to natural selection and involves a genetic change in the form of differing allele frequencies between generations.

Adaptation sensu lato describes any transgenerational process that enhances the fitness of the holobiont phenotype, and is comprised of (i) physiological transgenerational acclimatisation, (ii) host evolution, (iii) heritable microbial community changes and (iv) microbial evolution (the latter two components comprise the basis of microbiome-mediated transgenerational acclimatisation). Transgenerational acclimatisation on the host side often involves epigenetic changes.

Acclimatisation is one component of phenotypic plasticity describing the (often reversible) adjustment of an organisms’s physiology in response to a changing or novel environment.

Phenotypic plasticity (reversible, developmental or transgenerational) describes the ability of the same genotype to adjust its phenotype under different environmental conditions without genetic change. The reversible physiological component of plasticity corresponds to acclimatisation.

Epigenetics describes environmentally induced changes not encoded in the base sequence of the DNA that may nevertheless alter gene expression levels and have a heritable component. Among the possible mechanisms are DNA-methylation marks, histone acetylation and microRNAs.

Reaction norms describe the particular value of an organismal trait as a function of the environment, with the precise shape determining the plasticity of that trait.

The Price equation is an algorithm describing how the distribution of any characteristic (for example, genes, traits) changes in a biological population from one generation to the next. The basis of the Price equation: Δz=1/w cov(wi, zi)+1/w E(wi Δzi) is that a change in trait zz) is determined by the covariance between each value of z (zi) and fitness (wi), and the expected change in the trait value due to fitness (wi), (E(wi Δzi)). w represents mean fitness, E represents the population mean and cov represent the population covariance. The simple Price equation approach has been modified to a Price reaction norm approach (Govaert et al., 2016), and accomodates extinction and novel species within the community (Fox and Kerr, 2012).

Tolerance or phenotypic buffering describes how a genotype may function equally under ‘good’ and ‘adverse’ environmental conditions.

Phenotypic plasticity is the ability of the same genotype to adjust its phenotype under different environmental conditions without genetic change, with the reversible component of phenotypic plasticity corresponding to acclimatisation. ‘Classical’ within-generation acclimatisation involves an environmentally induced phenotypic response that alters individual fitness with no genetic change at the DNA level. An example would be acclimatisation within the same coral colony to different light levels (Ulstrup et al., 2006). There is increasing evidence however, that acclimatisation can also be transgenerational, for example, by altering chromatin structure or DNA methylation, which can also change gene expression (epigenetic changes, see Putnam et al., 2016). Acclimatisation can act during different ontogenetic stages, which distinguishes it from developmental plasticity that triggers non-reversible ontogenetic changes in particular phases of the life cycle (Gilbert et al., 2015).

Here, we stress that long-term acclimatisation of reef organisms may also be enabled via changes in the associated microbial species, which can evolve much more rapidly than the host, and which can also alter holobiont phenotype by frequency shifts and switching. Rapidly dividing symbiotic microbes (some bacteria divide every 20 min) are predicted to undergo adaptive evolution within weeks to months (Elena and Lenski, 2003), which is two to three orders of magnitude faster than genetic adaptation at the population level for the coral hosts. We call this mechanism microbiome-mediated acclimatisation, which if vertically transmitted would enable microbiome-mediated transgenerational acclimatisation (MMTA) of reef species. Microbially induced modifications of the host phenotype that alter host fitness and can be under selection pressure, has previously been proposed as part of the hologenome theory of evolution (Rosenberg and Zilber-Rosenberg, 2013). Here, we are particularly focussed on microbial contributions to transgenerational acclimatisation of reef species.

Functional importance of reef microbiomes

Recent molecular advances have revealed just how fundamentally important microorganisms are to the holobiont phenotype of almost all reef species. For instance, through translocation of photosynthetic assimilates and essential nutrients, the relationship between corals and their symbiotic dinoflagellates shape the health, tolerance limits and phenotypic plasticity of the coral holobiont. Similarly, the functional basis for most reef bacterial symbioses centres on cycling of essential nutrients such as carbon, nitrogen, sulphur and phosphate in addition to passage of trace metals, vitamin synthesis, provision of other cofactors and production of secondary metabolites (reviewed in (Bourne et al., 2016; Webster and Thomas, 2016)). At the other end of the interaction spectrum, colonisation by opportunistic and pathogenic microbes can result in diseased host phenotypes (Bourne et al., 2016).

Yet, despite the large body of literature describing the functional importance of microorganisms to the health and survival of reef species, to date, almost no research has explored the contribution of prokaryotic microorganisms to host transgenerational acclimatisation under changing environmental conditions. Although not a reef species, recent research on the microbiome of the model cnidarian Nematostella vectensis certainly suggests the microbiome plays a key role in host acclimatisation and maintenance of holobiont homeostasis under different environmental conditions (Mortzfeld et al., 2016). We know that future climate change conditions can rapidly alter the composition and function of reef host microbiomes (Supplementary Table 1 and reviewed in (Bourne et al., 2016; Webster and Thomas, 2016)), but whether these shifts in symbiosis are causally responsible for the ability of the host to acclimatise or adapt to new environmental conditions is still uncertain. Most researchers have focussed on a loss of symbionts correlating with declining host health. However, favourable symbiotic shifts that enhance the scope for growth or infer a competitive advantage to the host could also be passed to subsequent generations, ultimately enabling MMTA. Importantly, in addition to improved host fitness (Box 2), microbial symbionts may also confer environmental stress tolerance to their hosts (Dunbar et al., 2007), thus effectively modifying the ecological niche of the holobiont (Kopac and Klassen, 2016). The ‘coral probiotic hypothesis’ also posits that alterations in the coral microbiome may enable the host to avoid pathogenic infection under elevated seawater temperature (Reshef et al., 2006).

Box 2: Insights derived from other model systems:

The intensely studied human microbiome provides several analogous examples of the microbiome-mediated transgenerational acclimatisation concept. For example, a shift towards a diet high in simple carbohydrates in humans selected for a microbiome that could breakdown and absorb these compounds in the small intestine. Humans who outcompeted the microbes for these substrates had higher fitness, and this interaction between host genetics, environment (in this case diet) and the microbiome has ultimately shaped global human health (Walter and Ley, 2011). Similarly, there are numerous examples of how the human microbiome has adapted to our ecology. For example, a clear demarcation exists in the composition of gut Bacteroidetes between African and Western children, reflecting their contrasting diets. Local adaptation of the host can also occur via symbiont acquisition of novel genes, beautifully illustrated in the example of the porphyran-degrading gene acquired in Bacteroides plebeius from Japanese populations, enabling them to digest edible seaweed (Hehemann et al., 2010). It is anticipated that the insights derived from one of the most intensively studied model systems—humans—are just the tip of the iceberg with respect to discoveries of microbial contributions to host adaptation in complex microbiomes such as those occurring in reef holobionts.

Acclimatisation via symbiont shuffling

Variation in microbial symbiosis that contributes to local acclimatisation of the host can occur via microbial frequency shifts (symbiont shuffling), acquisition of novel microbial strains (symbiont switching) or horizontal gene transfer (HGT) between species (Figure 1). These processes can occur very rapidly when environmental conditions change, which may be an important element in the evolution of reef species. For instance, environmentally mediated microbial amplification was recently reported from a natural shallow water CO2 seep in Papua New Guinea (Morrow et al., 2015). In this environment, two species of reef sponge were found to be 40-fold more abundant at sites with active CO2 bubbling than they were at control sites only 100’s of metres away. Importantly, increased host abundance at the seep correlated with a significantly higher relative abundance of photosynthetic Synechococcus within the sponge holobionts at this site. This frequency shift of photosynthetic microorganisms at the seep likely provided the sponges with a greater nutritional benefit under future conditions of ocean acidification. Similarly, corals are known to harbour a diverse suite of symbiotic Symbiodinium that have distinct physiological optima, and shuffling the relative abundance of these symbionts within the holobiont provides corals with a mechanism to adjust their thermal tolerance (Baird et al., 2007). This community shift is evidenced by numerous studies documenting increased relative abundance of heat-tolerant Symbiodinium type D1 (LaJeunesse et al., 2014) in corals exposed to elevated seawater temperatures (Supplementary Table 1; (Howells et al., 2016); and references therein).

Figure 1
Figure 1

Conceptual overview of the microbial mechanisms facilitating transgenerational acclimatisation of coral reef organisms. Under elevated seawater temperature and pCO2, specific host-associated microorganisms may increase or decrease in abundance, thereby altering holobiont function and fitness. These frequency shifts can occur in the Symbiodinium (large circular cells differentially shaded according to clade) or prokaryotic community (rod-shaped cells). Shifts in community composition can also occur via acquisition of new microorganisms from the surrounding environment. In addition, mutation and/or horizontal gene transfer can introduce new genetic material into existing symbiont populations (white cells with black inset). This process can occur in any component (Symbiodinium, bacteria, archaea) of the host microbiome. If microbial alterations that infer a fitness advantage to the host are vertically transmitted to the offspring (denoted by sketch of a newly settled coral recruit), transgenerational acclimatisation can occur.

Acclimatisation via symbiont switching

Scleractinian corals are constantly exposed to a diverse suite of microorganisms present in their surrounding environment and, in some instances, these microbes may establish a new host niche enabling them to impact holobiont phenotype under altered environmental conditions. A compelling example is the selection of optimal coral algal symbionts from the surrounding environment depending on prevailing environmental conditions (Boulotte et al., 2016). Coral symbiont switching to a thermally resistant Symbiodinium clade was potentially driven by multiple preceding bleaching events (Boulotte et al., 2016). Switching of bacterial symbionts has also been observed in marine sponges and Foraminifera. For instance, when exposed to high-nutrient and low-salinity conditions over 12 months, the reef sponge Rhopaloeides odorabile replaces many of its native symbionts including the Poribacteria, with microorganisms having high similarity to symbionts of sponges known to inhabit more nutrient-rich environments (Webster et al., 2011). Similarly, the Foraminifera Heterostegina depressa switches between different Acidomicrobiales symbionts at different temperature and pCO2 levels (Webster et al., 2016). While MMTA still needs to be assessed for these examples, it is notable that both the sponges and Foraminifera are able to maintain their health for extended durations following symbiont switching (Webster et al., 2011), and the sponge species is also known to vertically transmit the majority of its complex microbiome (Webster et al., 2010). Importantly, symbiont shuffling and symbiont switching are not mutually exclusive; rather it is likely that both mechanisms operate in tandem. While the ability of reef organisms to associate with functionally diverse symbionts contributes to their success across a range of environmental conditions, assessments of heritable variation in tolerance are still required to estimate future evolutionary potential (Reusch, 2014).

Adaptation via genetic mutation within symbiont lineages

Because of their rapid division rates, symbiotic microbes are much more prone to adaptive evolution within their mitotically dividing and sexual phases than their eukaryotic hosts. Sexual recombination in Symbiodinium has recently been suggested based on the analysis of DNA polymorphisms (Wilkinson et al., 2015) and genome analysis revealing numerous meiosis-specific genes (Chi et al., 2014). However, direct observational evidence or single-cell genotyping approaches that target multiple, single-copy loci are still required for conclusive evidence for inter-lineage recombination and introgression. Regardless of the mechanism, Symbiodinium populations have been shown to influence holobiont tolerance through physiological and genetic adaptation to prevailing conditions (Supplementary Table 1; (Levin et al., 2016); and references therein). Several physiological and transcriptional differences (related to photosynthetic efficiency, reactive oxygen species and molecular chaperones) exist between thermo-sensitive C1 and thermo-tolerant C1 Symbiodinium populations that possibly have a genetic basis. Both populations display an enrichment of meiosis functional gene groups at elevated temperature, which is important in promoting adaptation (Levin et al., 2016) compared to purely asexually dividing strains (Kaltz and Bell, 2002). Adaptive variation in thermal tolerance of asexual Symbiodinium C1 cultures is also retained following a year of growth at lower temperatures (Howells et al., 2012). A novel experimental evolution study recently confirmed the possibility of adaptive evolution enhancing thermal tolerance in Symbiodinium symbionts ex-hospite (Chakravarti et al., 2017). Significant adaptive potential therefore exists if the rates of somatic mutation in Symbiodinium are found to be similar to other species of single-celled eukaryotic algae (Costas et al., 2008), especially considering their fast asexual generation times and large population sizes within corals. Furthermore, elevated seawater temperatures associated with global climate change likely provide strong selective pressure favouring the persistence and proliferation of thermally tolerant genetic variants. Nothing is known thus far on ‘within-host’ evolution of associated bacteria, yet they may evolve even faster than eukaryotic microbes because of ample mechanisms of gene acquisition via HGT (see below) and since any mutation is immediately visible to selection in haploid bacteria and archaea.

Adaptation via HGT

The mutational basis of genomic change within microbial populations may be greatly enhanced by mechanisms of HGT. Conjugation and transformation provide mechanisms for modification of bacterial DNA with inter-species ‘mating’ and natural competence being two major mechanisms driving HGT in bacteria. Most microorganisms also possess different classes of mobile genetic elements that allow for the acquisition, loss or rearrangement of regions of the bacterial genome. For instance, recent analysis of the human microbiome revealed pervasive mobile genes with functional variation across human populations corresponding to known dietary differences (Chi et al., 2014), highlighting environment as a strong selective pressure in shaping mobile gene pools. In addition, viruses can shuttle these genes between different components of the holobiont via a third HGT mechanism, transduction (Hambly and Suttle, 2005). Viral-mediated HGT of beneficial genes (between different symbiotic microorganisms and/or between host and symbionts) has the potential to increase host fitness and cause rapid ecological speciation that allows reef holobionts to adapt and survive under changing environmental conditions. While HGT among prokaryotes and viruses is ubiquitous, cases of HGT among eukarya/metazoan hosts and prokaryotes are less clear. One excellent example of suspected HGT in sponges involves a spherulin-encoding gene being transferred from a microorganism to the host (Jackson et al., 2011). Spheruline is thought to be involved in skeletogenesis, indicating that this HGT event may have contributed to the evolution of the sponge body plan. Another example, is the suspected transfer of eukaryotic-like proteins from the sponge host to the symbionts, with these eukaryotic-like proteins thought to be used by the symbionts to regulate host phagocytosis (Nguyen et al., 2014). These examples demonstrate the potential for HGT in reef holobionts, and future examination of existing and emerging reef genomic data (such as that being generated by the ReFuGe 2020 consortium; (Voolstra et al., 2015)) is expected to reveal more specific examples related to environmental adaptation.

A conceptual framework for predicting holobiont acclimatisation and adaptation

To assess the relative importance of the mechanisms leading to adaptation in reef organisms via MMTA, we recommend employing the Price equation (Price, 1970). The Price equation has been applied in community ecology to disentangle the relative importance of within-species changes owing to plasticity based on genetic adaptation and community change, for the functioning (for example, biomass production) of that community (Collins and Gardner, 2009; Govaert et al., 2016). This is achieved by viewing the holobiont’s microbial community as plastic, describing how the distribution (for example, mean, variance, and so on) of any characteristic (for example, genes, trait values, and so on) changes in a biological population between generations. The Price equation partitions phenotypic responses into (i) physiological, (ii) ecological and (iii) evolutionary components, and this quantitative framework therefore provides a conceptual tool for understanding and predicting the acclimatisation and adaptation of the holobiont to global change. For instance, the composition/function (i—physiological) and abundance (ii—ecological) of a coral-associated microbiome may undergo rapid shifts as environmental conditions change, and microbial lineages that survive exposure to elevated seawater temperature and/or ocean acidification may also undergo microevolution (iii—evolutionary), all of which ultimately impacts the holobiont phenotype under climate change. Note that the compositional term integrates two processes, the acquisition and local extinction of symbionts and shifts in relative frequency of the original community (Fox and Kerr, 2012).

The Price equation approach has recently been successfully combined with reaction norm analysis (relating mean phenotypic response of a genotype to the change in environment) (Govaert et al., 2016). This combined Price equation/reaction norm metric should successfully disentangle non-evolutionary and evolutionary contributors, and their interaction to trait change (phenotypic plasticity, species sorting and the interaction between species sorting and evolutionary components) within reef holobionts. Note that currently, the Price equation approach is only strictly valid for traits as state variables, for example, contributions to the energy allocation of a coral polyp. Depending on the response variable upon environmental disturbance, modifications of the partitioning approach would need to be developed. For instance, if rates (for example, photosynthesis rates) were of interest, current approaches would need to be modified to represent such variables. Although it may be difficult in complex natural systems to measure all required components of within- and among microbial symbiont change, it is nevertheless conceptually useful and may guide simplified experiments to integrate MMTA across levels of biological organisation. We also suggest broadening the Price equation/reaction norm concept by allowing for genetic exchange within the microbiome via HGT.

Hologenome concept

Adaptation sensu lato as discussed here encompasses any fitness gain of the collective phenotype under novel stressful conditions. In line with the emerging consensus on the hologenome theory and levels of selection (Bordenstein and Theis, 2015; Moran and Sloan, 2015; Kopac and Klassen, 2016; Douglas and Werren, 2016; Theis et al., 2016), this does not require co-evolution of the entire holobiont as an individual unit. Rather, specific microorganisms or microbial genes that provide a fitness advantage to the host under conditions of elevated sea temperature and ocean acidification could be maintained and passed to subsequent generations, ultimately resulting in transgenerational acclimatisation of the host. For instance, it has been established that corals can effectively regulate their internal pH enabling them to at least partially mitigate the effects of ocean acidification (McCulloch et al., 2012). However, the physiological or energetic cost of this response may only be sustainable in the longer term if there is a shift in the symbiotic microbiome towards a community that meets the increased energetic demand. The holobiont would not only be better acclimatised to its local environment, but would also have an increased probability of passing on the acquired microbial traits to the next generation. Thus, the individual evolutionary trajectories of the different holobiont members can still enable transgenerational acclimatisation of a reef organism, regardless of whether the holobiont is heritable as a unit. Reef holobionts can assemble their diverse microbiomes to maximise host fitness, and then environmental forces can select for reassembly or new membership as required.

Symbiont transmission facilitating transgenerational acclimatisation

For microbial species to play a role in MMTA, they must be faithfully transmitted between generations (vertical transmission) or dependably acquired from the environment in each new generation (horizontal transmission). A major consideration in the hologenome debate is that higher levels of selection are most likely to apply to particular host-symbiont pairs with strict partner fidelity rather than applying to the entire microbiome. Variation in partner fidelity undoubtedly occurs across the complex microbiomes of reef species such as corals and sponges, and it is highly unlikely that the entire microbiome of a reef organism would evolve as a holobiont unit with mutually beneficial coadaptation. However, in a large number of reef species there is support for high partner fidelity in at least some components of the complex microbiome, with particular microorganisms inhabiting corals, sponges and ascidians being vertically transmitted (Supplementary Table 1 and reviewed in (Bourne et al., 2016; Webster and Thomas, 2016)). Some reef species also reproduce asexually via fragmentation or production of asexual gemmules, so that each of the fragments inherits the same microbiome as the adult population. However, MMTA is unlikely to be ubiquitous across all reef species as it can only occur if there is strict vertical transmission of the altered microbiome, and some coral species actually obtain new symbionts from the surrounding environment each generation. For instance, recent analysis of coral and algal microbiomes revealed communities of highly diverse and stable symbionts, but no single bacterial species that was ubiquitously associated with a single host species, indicating a lack of strict symbiont heredity (Hester et al., 2016). Unfortunately, however, in most reef species, there is still little understanding of how stringent the vertical transmission process is, and whether these populations persist or change during larval metamorphosis and subsequent juvenile development.

Functional equivalence and evolutionary convergence in reef microbiomes

Until recently, it was also unknown whether the diverse microbiomes of reef species shared functions, and if so, whether these equivalent functions were undertaken by evolutionary convergent mechanisms (Webster and Thomas, 2016). However, recent research on sponge microbiomes found that despite the species-specific taxonomic profiles that are characteristic of sponges (Thomas et al., 2016), core functions such as nitrogen metabolism and nutrient utilisation were shared across phylogenetically disparate microorganisms (Fan et al., 2012; Ribes et al., 2012). Microbial communities in divergent hosts seemingly evolved different genomic solutions to undertake the same function or inhabit the same niche with many of these core symbiont functions being provided by equivalent enzymes and biosynthetic pathways (Fan et al., 2012). Even when free-living microorganisms are initially able to enter into low selectivity or random (that is, neutral) partnerships with the host, as the symbiotic relationship evolves and vertical transmission occurs, symbionts should maintain or acquire functions that stabilise their interaction with their host. Thus, for different host species with similar functional niches, symbionts will eventually converge functionally and the collective gene pool encompassed within the holobiont is therefore critical for transgenerational acclimatisation of the host.


We propose here an inclusive concept of adaptation sensu lato in coral reef organisms, that encompasses in addition to (epi)genetic adaptation of the host, a pervasive role of MMTA via symbiont shuffling and switching, genetic mutation, and horizontal transfer of beneficial genes that are inherited by offspring. Successful modelling of future reefs therefore requires an accurate assessment of several as yet understudied holobiont processes (Figure 1). To date, most experimental research has focussed on determining the phenotypic responses of reef organisms in single-factor, short-term, single generation experiments, severely limiting our ability to assess the potential for evolutionary adaptation. Recommendations for future research on reef species therefore include (i) determining if variation in host fitness due to assembly of different microbiomes with unique microbial functions ultimately drives a multigenerational response to selection, (ii) experimentally assessing the mechanisms and rates of transgenerational acclimatisation and adaptation, (iii) manipulative experiments to alter microbial composition and assess phenotypic responses, (iv) determining the extent of functional equivalence in microbiomes to assess whether hidden diversity is critical for holobiont functioning under ocean change, and (v) using a modified Price equation (Price, 1970; Govaert et al., 2016) to integrate the relative contributions of (epi)genetic adaptations in the host via (a) germ line and mitotic mutations, (b) microbiome changes established by both shuffling and switching and (c) evolution within microbial strains including HGT.


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We acknowledge the many pertinent studies on reef adaptation that could not be included in this article because of space restrictions. NSW was funded through an Australian Research Council Future Fellowship FT120100480 and TBHR acknowledges funding from the DFG collaborative research centre CRC1182 ‘Origin and Function of Metaorganisms’. Tim Simmonds from the Australian Institute of Marine Science is gratefully acknowledged for his graphical design expertise applied in Figure 1.

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  1. Australian Institute of Marine Science, Townsville, Queensland, Australia

    • Nicole S Webster
  2. Australian Centre for Ecogenomics, University of Queensland, Brisbane, Queensland, Australia

    • Nicole S Webster
  3. GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany

    • Thorsten B H Reusch


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Correspondence to Nicole S Webster.

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