Abstract
Objective:
To investigate the effect of glucose and insulin concentrations on differentiation of umbilical cord lining progenitor cells to adipocyte-like cells (ALCs).
Methods:
Cord lining mesenchymal cells (CLMCs) were isolated from the explant of human umbilical cord amniotic membrane. CLMCs were subjected to differentiation under various culture conditions for 20 days. Lipid droplets were confirmed with Oil Red O staining. Gene expressions of adipsin and peroxisome proliferator-activated receptor gamma (PPARĪ³) were analyzed using reverse transcription-PCR. Leptin and adiponectin secretions were detected using enzyme-linked immunosorbent assay kit.
Results:
CLMCs became irregular, cuboidal-shaped cells that resemble adipocytes, and Oil Red O staining showed the presence of lipid droplets. The gene expressions of PPARĪ³ and adipsin were upregulated. Leptin and adiponectin secretions by naive CLMCs were below the limits of detection. Matured ALCs cultured in low-glucose medium significantly secreted leptin and adiponectin, whereas those in high-glucose medium significantly secreted only leptin. Insulin concentration affects leptin but not adiponectin secretion.
Conclusions:
Under different culture conditions, CLMCs can differentiate into ALCs that resemble adipocytes in either normal-weight or obese individuals. Hence, these ALCs have the potential to be used as an in vitro model to study adipogenesis and obesity, and possibly as a drug discovery model for metabolic disorders.
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Acknowledgements
This work was supported by the research scholarship awarded to Han Hui Cheong and the research support from the National University of Singapore to Sui Yung Chan. We thank CellResearch Corporation, Singapore for the generous gift of cord lining mesenchymal cells and proprietary expansion medium. And also thank Dr Perry Fung Chye Lim and Dr Lifeng Kang for their advice on statistical analysis. The material in this study is an original research and has not been previously published.
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Dr Phan Toan Thang is the chief research scientist at the CellResearch Corporation, Singapore.
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This work was presented as a poster at the 2009 AAPS Annual Meeting and Exposition, held at the Los Angeles Convention Centre on 8ā12 November 2009.The preliminary data of this work were presented as a poster at the JAMA-NUHS CME conference āRecent Trends in Diabetes, Obesity, and Metabolic Syndromeā held on 1ā2 August 2008 at the University Cultural Hall, Singapore.
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Cheong, H., Masilamani, J., Phan, T. et al. Cord lining progenitor cells: potential in vitro adipogenesis model. Int J Obes 34, 1625ā1633 (2010). https://doi.org/10.1038/ijo.2010.86
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DOI: https://doi.org/10.1038/ijo.2010.86
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