Abstract
Background:
Recent findings regarding the existence of functional brown adipose tissue (BAT) in adult humans suggest a physiological role of BAT and uncoupling protein 1 (UCP1)–linked thermogenesis in energy balance.
Objective:
To investigate whether UCP1 polymorphism was associated with resting energy expenditure (REE) and thermoregulatory sympathetic nervous system (SNS) activity in humans.
Methods:
A total of 82 healthy females (20–22 years) were genotyped for the –3826 A/G polymorphism of the UCP1 gene using a fluorescent allele-specific DNA primer assay system. REE was measured by indirect calorimetry. The thermoregulatory SNS activity was assessed by heart rate variability power spectral analysis according to our previously reported method. Each subject was studied in the morning, after an overnight fast. Nutritional values were calculated on the basis of 2-day food records.
Results:
The frequencies of A/A, A/G and G/G genotypes were 0.27, 0.45 and 0.28, respectively. No significant difference was found in anthropometric indexes among the three groups. However, in the G/G group, the percentage of energy consumed as fat was lower (A/A: 30.7±1.1%, A/G: 31.3±1.0%, G/G: 26.0±1.2%, P<0.01), and energy intake tended to be lower (A/A: 7209±310 kJ d−1, A/G: 7075±280 kJ d−1, G/G: 6414±264 kJ d−1, P=0.16). With regard to metabolic parameters, group differences were observed in REE (A/A: 5599±170 kJ d−1, A/G: 5054±115 kJ d−1, G/G: 4919±182 kJ d−1, P<0.01) and in thermoregulatory SNS activity (A/A: 313±47 ms2, A/G: 333±42 ms2, G/G: 185±23 ms2, P<0.05).
Conclusion:
Diminished REE in G-allele carriers as well as reduced thermoregulatory SNS activity for the G/G genotype, suggest that attenuated UCP1-linked thermogenesis has an adverse effect on the regulation of energy balance.
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References
Saito M, Okamatsu-Ogura Y, Matsushita M, Watanabe K, Yoneshiro T, Nio-Kobayashi J et al. High incidence of metabolically active brown adipose tissue in healthy adult humans: effects of cold exposure and adiposity. Diabetes 2009; 58: 1526–1531.
van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM, Drossaerts JM, Kemerink GJ, Bouvy ND et al. Cold-activated brown adipose tissue in healthy men. N Engl J Med 2009; 360: 1500–1508.
Cypess AM, Lehman S, Williams G, Tal I, Rodman D, Goldfine AB et al. Identification and importance of brown adipose tissue in adult humans. N Engl J Med 2009; 360: 1509–1517.
Virtanen KA, Lidell ME, Orava J, Heglind M, Westergren R, Niemi T et al. Functional brown adipose tissue in healthy adults. N Engl J Med 2009; 360: 1518–1525.
Zingaretti MC, Crosta F, Vitali A, Guerrieri M, Frontini A, Cannon B et al. The presence of UCP1 demonstrates that metabolically active adipose tissue in the neck of adult humans truly represents brown adipose tissue. FASEB J 2009; 23: 3113–3120.
Lowell BB, Spiegelman BM . Towards a molecular understanding of adaptive thermogenesis. Nature 2000; 404: 652–660.
Cannon B, Nedergaard J . Brown adipose tissue: function and physiological significance. Physiol Rev 2004; 84: 277–359.
Jia JJ, Tian YB, Cao ZH, Tao LL, Zhang X, Gao SZ et al. The polymorphisms of UCP1 genes associated with fat metabolism, obesity and diabetes. Mol Biol Rep 2010; 37: 1513–1522.
Esterbauer H, Oberkofler H, Liu YM, Breban D, Hell E, Krempler F et al. Uncoupling protein-1 mRNA expression in obese human subjects: the role of sequence variations at the uncoupling protein-1 gene locus. J Lip Res 1998; 39: 834–844.
Nagai N, Sakane N, Ueno LM, Hamada T, Moritani T . The -3826 A → G variant of the uncoupling protein-1 gene diminishes postprandial thermogenesis after a high fat meal in healthy boys. J Clin Endocrinol Metab 2003; 88: 5661–5667.
Nagai N, Sakane N, Fujishita A, Fujiwara R, Kimura T, Kotani K et al. The -3826 A → G variant of the uncoupling protein-1 gene diminishes thermogenesis during acute cold exposure in healthy children. Obes Res Clin Pract 2007; 1: 99–107.
Matsumoto T, Miyawaki T, Ue H, Zenji C, Moritani T . Autonomic responsiveness to acute cold exposure in obese and non-obese young women. Int J Obes Relat Metab Disord 1999; 23: 793–800.
Matsumoto T, Miyawaki C, Ue H, Kanda T, Yoshitake Y, Moritani T . Comparison of thermogenic sympathetic response to food intake between obese and non-obese young women. Obes Res 2001; 9: 78–85.
Matsumoto T, Miyawaki C, Ue H, Yuasa T, Miyatsuji A, Moritani T . Effects of capsaicin-containing yellow curry sauce on sympathetic nervous system activity and diet-induced thermogenesis in lean and obese young women. J Nutr Sci Vitaminol 2000; 46: 309–315.
Nagai N, Sakane N, Hamada T, Kimura T, Moritani T . The effect of a high-carbohydrate meal on postprandial thermogenesis and sympathetic nervous system activity in boys with a recent onset of obesity. Metabolism 2005; 54: 430–438.
Nagai N, Sakane N, Narukami H, Kugenuma H, Moritani T . The effect of pungent principle of spices on postprandial thermogenesis, satiety, and sympathetic nervous system activity in children. Jap Soc Study Obes 2003; 9: 156–163 (in Japanese).
Resources Council, Ministry of Education, Culture, Sports, Science, Technology of Japan. The Japanese food composition table. 5th revision. Tokyo, Daiichi Publishing Co.: Japan, 2009 (in Japanese).
Lusk G . Animal calorimetry: analysis of the oxidation of mixtures of carbohydrate and fat. J Biol Chem 1924; 59: 41–42.
Akselrod S, Gordon D, Ubel FA, Shannon DC, Barger AC, Cohen RJ . Power spectrum analysis of heart rate fluctuation: a quantitative prove of beat-to-beat cardiovascular control. Science 1981; 213: 220–222.
Yoneshiro T, Aita S, Matsushita M, Kameya T, Nakada K, Kawai Y et al. Brown adipose tissue, whole-body energy expenditure, and thermogenesis in healthy adult men. Obesity 2010. doi:10.1038/oby.2010.105.
Nagai N, Sakane N, Moritani T . Impact of aging and β3-Adrenergic-receptor polymorphism on thermic and sympathetic responses to a high-fat meal. J Nutr Sci Vitaminol 2006; 52: 352–359.
Seale P, Lazar MA . Brown fat in humans: turning up the heat on obesity. Diabetes 2009; 58: 1482–1484.
Nagai N, Sakane N, Moritani T . Effect of skipping breakfast and macro-nutrient balance on postprandial blood glucose, satiety, energy expenditure, and autonomic nervous system activity in healthy young subjects. J Japan Diab Soc 2005; 48: 761–770 (in Japanese).
Kobashi R, Wakisaka S, Hayashi N, Sakane N, Moritani T, Nagai N . Influence of diet experience on autonomic nervous system activity in young females. Jap Soc Study Obes 2009; 15: 179–184 (in Japanese).
Lee P, Greenfield JR, Ho KK, Fulham MJ . A critical appraisal of the prevalence and metabolic significance of brown adipose tissue in adult humans. Am J Physiol Endocrinol Metab 2010; 299: E601–E606.
Pfannenberg C, Werner MK, Ripkens S, Stef I, Deckert A, Schmadl M et al. Impact of age on the relationships of brown adipose tissue with sex and adiposity in humans. Diabetes 2010; 59: 1789–1793.
Matsushita H, Kurabayashi T, Tomita M, Kato N, Tanaka K . Effects of uncoupling protein and β3-adrenergic receptor gene polymorphisms on body size and serum lipid concentrations in Japanese women. Maturitas 2003; 45: 39–45.
Acknowledgements
We are grateful to all subjects for their cooperation. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (21500786).
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Nagai, N., Sakane, N., Tsuzaki, K. et al. UCP1 genetic polymorphism (–3826 A/G) diminishes resting energy expenditure and thermoregulatory sympathetic nervous system activity in young females. Int J Obes 35, 1050–1055 (2011). https://doi.org/10.1038/ijo.2010.261
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DOI: https://doi.org/10.1038/ijo.2010.261
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