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Blood orange juice inhibits fat accumulation in mice



To analyze the effect of the juice obtained from two varieties of sweet orange (Citrus sinensis L. Osbeck), Moro (a blood orange) and Navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (HFD).


Obesity was induced in male C57/Bl6 mice by feeding a HFD. Moro and Navelina juices were provided instead of water. The effect of an anthocyanin-enriched extract from Moro oranges or purified cyanidin-3-glucoside (C3G) was also analyzed. Body weight and food intake were measured regularly over a 12-week period. The adipose pads were weighted and analyzed histologically; total RNA was also isolated for microarray analysis.


Dietary supplementation of Moro juice, but not Navelina juice significantly reduced body weight gain and fat accumulation regardless of the increased energy intake because of sugar content. Furthermore, mice drinking Moro juice were resistant to HFD-induced obesity with no alterations in food intake. Only the anthocyanin extract, but not the purified C3G, slightly affected fat accumulation. High-throughput gene expression analysis of fat tissues confirmed that Moro juice could entirely rescue the high fat-induced transcriptional reprogramming.


Moro juice anti-obesity effect on fat accumulation cannot be explained only by its anthocyanin content. Our findings suggest that multiple components present in the Moro orange juice might act synergistically to inhibit fat accumulation.

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We thank Elena Beltrami for many helpful discussions and Paola Dalton for the help in writing the paper. We thank also Ivan Toschi, Valentina and Nicoletta Cesari for the help in energy expenditure analysis and Simone Paolo Minardi for the assistance in the Affymetrix analysis. This work was supported by the EU FP6 FLORA project (FOOD-CT-01730) awarded to MG, CT, PGP, SM and GRR. This work was financially supported by EU FP6 FLORA project (FOOD-CT-01730).

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Correspondence to M Giorgio.

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Titta, L., Trinei, M., Stendardo, M. et al. Blood orange juice inhibits fat accumulation in mice. Int J Obes 34, 578–588 (2010).

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  • blood orange
  • orange juice
  • fat accumulation
  • anti-obesity
  • anthocyanins
  • cyanidin-3-glucoside.

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