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NOD/SCID repopulating cells contribute only to short-term repopulation in the baboon

Gene Therapy volume 15, pages 1460–1462 (2008)Cite this article

Abstract

We have previously compared the repopulation ability of gene-modified baboon CD34+ cells in an autologous transplantation versus a xenotransplant model in irradiated nonobese diabetic/severe combined immune deficiency (NOD/SCID) mice. Baboon CD34-selected marrow cells were transduced with a gammaretrovirus vector and infused into irradiated baboons and NOD/SCID mice. A limited integration-site analysis could only detect two common retrovirus integration sites in the NOD/SCID and monkey. Here, we performed locus-specific PCR on 30 clones recovered from NOD/SCID β2-microglobulin mice reconstituted with transduced baboon CD34+ cells. We identified five common integrants in the baboon early after transplant (2–6 weeks) but none during the long-term follow-up (6 and 12 months). These results confirm that repopulating cells in the NOD/SCID mouse contribute only to short-term repopulation in a clinically relevant large animal model.

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Authors and Affiliations

  1. Fred Hutchinson Cancer Research Center, Clinical Research Division, Seattle, WA, USA

    P Mezquita, B C Beard & H-P Kiem

  2. University of Washington School of Medicine, Seattle, WA, USA

    H-P Kiem

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  1. P Mezquita
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  2. B C Beard
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  3. H-P Kiem
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Correspondence to H-P Kiem.

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Mezquita, P., Beard, B. & Kiem, HP. NOD/SCID repopulating cells contribute only to short-term repopulation in the baboon. Gene Ther 15, 1460–1462 (2008). https://doi.org/10.1038/gt.2008.108

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  • DOI: https://doi.org/10.1038/gt.2008.108

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