Original Article | Published:

Direct measurement of B-cell receptor repertoire’s composition and variation in systemic lupus erythematosus

Genes and Immunity volume 18, pages 2227 (2017) | Download Citation

Abstract

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that is known to be associated with polyclonal B-cell hyper-reactivity. B-cell receptor (BCR) has a central role in B-cell development, activation, survival and apoptosis, and thus is a critical component of the regulation of both protective and autoreactive B cells. In this study, we applied multiplex PCR and Illumina high-throughput sequencing to study the composition and variation of the BCRs in peripheral blood mononuclear cells from SLE patients and healthy donors (NC). We found that SLE group displayed significantly shorter CDR3 average length (14.86±0.76aa vs 15.70±0.43aa), more arginine percentage of CDR3 amino acids (7.57±0.20% vs 7.32±0.19%) and poorer immunological diversity than the healthy ones. CDR3 sequence YGMDV present in all SLE samples may provide more information in generating more effective B-cell targeted diagnosis/therapies strategies.

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Acknowledgements

This work was supported by funds received from Science and Technology Plan of Shenzhen, Guangdong (No. JCYJ20160422150329190), the fund received from National Natural Science Foundation of China (No. 81671596).

Author information

Affiliations

  1. Department of Clinical Medical Research, The Second Clinical Medical College of Jinan University (Shenzhen People’s Hospital), Shenzhen, China

    • S Liu
    •  & Y Dai
  2. Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, China

    • X L Hou
    •  & W G Sui
  3. Department of Dermatology, Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, China

    • Q J Lu
  4. Department of Cancer Research, Shenzhen University, Shenzhen, China

    • Y L Hu
  5. Department of Basic Medical Sciences, Wuhan University, Wuhan, China

    • Y L Hu

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Competing interests

The authors declare no conflict of interest.

Corresponding author

Correspondence to Y Dai.

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DOI

https://doi.org/10.1038/gene.2016.45

Supplementary Information accompanies this paper on Genes and Immunity website (http://www.nature.com/gene)

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