Abstract
Immunoglobulin (Ig) genes specifically recruit activation-induced deaminase (AID) for ‘on-target’ DNA deamination, initiating either variable (V) region somatic hypermutation, or double-strand break intermediates of class switch recombination (CSR). Such breaks overwhelmingly undergo legitimate intra-Ig repair rather than rare illegitimate and potentially oncogenic junctions outside of Ig loci. We show that in human B cells, legitimate synapsis and repair efficiently join Ig genes whether physically linked on one chromosome or located apart on both alleles. This indicates mechanisms faithfully recognizing and/or pairing loci with homology in structure and accessibility, thus licensing interchromosomal trans-CSR junctions while usually preventing illegitimate interchromosomal recombination with AID off-target genes. Physical linkage of IgH genes in cis on the same allele just increases the likelihood of legitimate repair by another fourfold. The strongest force driving CSR might thus be recognition of legitimate target genes. Formation of IgH intra-allelic loops along this process would then constitute a consequence rather than a pre-requisite of this gene-pairing process.
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Acknowledgements
This work was supported by grants from Association pour la Recherche sur le Cancer (ARC SL 220100601332), ANR (IgHAccess 2011 BSV302701). Ligue Nationale contre le Cancer and Conseil Régional du Limousin. BL and SMB were supported by Association pour la Recherche sur le Cancer.
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Laffleur, B., Bardet, S., Garot, A. et al. Immunoglobulin genes undergo legitimate repair in human B cells not only after cis- but also frequent trans-class switch recombination. Genes Immun 15, 341–346 (2014). https://doi.org/10.1038/gene.2014.25
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DOI: https://doi.org/10.1038/gene.2014.25