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Interleukin-20 induced cell death in renal epithelial cells and was associated with acute renal failure

Genes & Immunity volume 9, pages 395–404 (2008)Cite this article

Abstract

Acute renal failure is an abrupt decrease in renal function. Interleukin (IL)-10 inhibits ischemic and cisplatin-induced acute renal failure. We aimed to determine whether IL-20 affects renal tubular epithelial cells and is associated with acute renal failure. We analyzed the expression of IL-20 and its receptor (R) in the kidneys of rats with HgCl2-induced acute renal failure. Reverse transcription-PCR showed upregulated IL-20, and its receptors and immunohistochemical staining showed strongly expressed IL-20 protein in proximal tubular epithelial cells. We analyzed human proximal tubular epithelial (HK-2) cells, which expressed both IL-20 and its receptors. IL-20 specifically induced mitochondria-dependent apoptosis by activating caspase 9 in HK-2 cells. IL-20 also activated c-Jun N-terminal kinase and extracellular signal-regulated kinase 1/2, the downstream signals implicated in the apoptosis of HK-2 cells. Furthermore, IL-20 upregulated the transcripts of transforming growth factor (TGF)-β1, a critical mediator of renal injury. In hypoxic HK-2 cells, IL-20 and IL-22R1 transcripts increased, and IL-20 upregulated IL-1β transcripts. In vivo study further demonstrated that anti-IL-20 antibody reduced the expression of TGF-β1 and IL-1β and the number of damaged tubular cells in the kidneys of rats with acute renal failure. We concluded that IL-20 may be involved in the injury of renal epithelial cells in acute renal failure.

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Authors and Affiliations

  1. Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan

    H-H Li, C-C Wei & M-S Chang

  2. Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan

    H-H Li & M-S Chang

  3. Institute of Biopharmaceutical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan

    Y-H Hsu & M-S Chang

  4. Department of Medical Technology, Chung Hwa University of Medical Technology, Tainan, Taiwan

    C-C Wei

  5. Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan

    P-T Lee

  6. Division of Nephrology, Department of Medicine, Kaohsiung Veterans General Hospital, Kaohsiung & National Yang-Ming University, School of Medicine, Taipei, Taiwan

    P-T Lee

  7. Department of Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan

    W-C Chen

  8. Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan

    M-S Chang

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  1. H-H Li
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Correspondence to M-S Chang.

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This work was supported by a grant from Chi Mei Medical Center, Tainan, Taiwan.

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The authors declare no conflict of interests.

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Li, HH., Hsu, YH., Wei, CC. et al. Interleukin-20 induced cell death in renal epithelial cells and was associated with acute renal failure. Genes Immun 9, 395–404 (2008). https://doi.org/10.1038/gene.2008.28

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