Abstract
Purpose To study the effect of Nd:YAG laser trabeculoplasty (LT) on endothelin-1 (ET-1) concentration of aqueous humour and intraocular pressure (IOP) in rabbits.
Methods One eye of each of 18 pigmented rabbits was subjected to 360° LT with a frequency-doubled Nd:YAG laser (532 nm), and IOP was measured with a Tono-Pen tonometer before treatment. Post-LT IOP measurements followed by aqueous humour aspiration were performed under general anaesthesia at 3 and 12 h and 1, 3 and 7 days after the treatment. The concentration of ET-1 in aqueous humour was measured by means of a radioimmunoassay.
Results In the eyes that had undergone LT, the concentrations of ET-1 in the aqueous humour were significantly increased at 3,12 and 24 h after the treatment compared with the contralateral eyes. ET-1 concentrations at 3 and 7 days after LT, however, did not differ significantly from the corresponding contralateral control values. IOP increased following the treatment at 3 and 12 h. IOP values were significantly lower in the treated eyes at 1, 3 and 7 days after the treatment than in the control eyes.
Conclusions The results show that LT in rabbits was followed by an immediate and short-term increase in aqueous humour ET-1 that might be caused by leakage from uveal tissue. This may be responsible for the release of prostaglandins causing the IOP increase and inflammatory complications of LT.
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Guzey, M., Vural, H. & Satici, A. Endothelin-1 increase in aqueous humour caused by frequency-doubled Nd:YAG laser trabeculoplasty in rabbits. Eye 15, 781–785 (2001). https://doi.org/10.1038/eye.2001.249
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DOI: https://doi.org/10.1038/eye.2001.249