In vitro corneal pathogenicity of Acanthamoeba

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The comparative cytopathic effects of a keratitis and an environmental isolate of Acanthamoeba were studied on confluent monolayers of human and rabbit corneal cells grown in culture. The presence of cells in culture induced excystment of amoebae to the active trophozoite form. Total destruction of cell monolayers was observed to be similar for both isolates, and dependent on incubation time and amoebic concentration. The relevance of these findings to human and experimental Acanthamoeba keratitis is discussed.


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Correspondence to D F P Larkin.

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  • Acanthamoeba
  • cytopathogenicity
  • keratocytes

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