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Sleep, circadian rhythms, and the pathogenesis of Alzheimer Disease

Experimental & Molecular Medicine volume 47, page e148 (2015) | Download Citation

Abstract

Disturbances in the sleep–wake cycle and circadian rhythms are common symptoms of Alzheimer Disease (AD), and they have generally been considered as late consequences of the neurodegenerative processes. Recent evidence demonstrates that sleep–wake and circadian disruption often occur early in the course of the disease and may even precede the development of cognitive symptoms. Furthermore, the sleep–wake cycle appears to regulate levels of the pathogenic amyloid-beta peptide in the brain, and manipulating sleep can influence AD-related pathology in mouse models via multiple mechanisms. Finally, the circadian clock system, which controls the sleep–wake cycle and other diurnal oscillations in mice and humans, may also have a role in the neurodegenerative process. In this review, we examine the current literature related to the mechanisms by which sleep and circadian rhythms might impact AD pathogenesis, and we discuss potential therapeutic strategies targeting these systems for the prevention of AD.

Introduction

Though sleep consumes a considerable portion of our lives, its biological functions and biochemical implications are still poorly understood. Efficient and effective sleep is more than just a luxury; it is critical for the proper function of many organ systems, particularly the brain. The sleep–wake cycle is the most obvious example of a circadian process, as it occurs with a consistent 24-h rhythm and can be shifted according to environmental cues. The body’s circadian clock system maintains these 24-h rhythms in physiological functions, including the sleep–wake cycle, and synchronizes them to the light–dark cycle. Disturbances of both sleep and the underlying circadian rhythms have long been associated with many neurological and psychiatric diseases, including Alzheimer Disease (AD). AD, a devastating age-related neurodegenerative disease characterized by aggregation and accumulation of amyloid-β (Aβ) and tau proteins, is the most common cause of dementia in older people worldwide. AD patients often exhibit disrupted day–night activity patterns and fragmented or mistimed sleep, which can cause great morbidity and is a major cause of institutionalization. Although these sleep and circadian abnormalities were once dismissed as consequences of the disease process, accumulating evidence suggests that sleep and circadian disturbances likely occur very early in the disease process and may contribute to the pathogenesis of AD. Thus, the possibility exist that treating the sleep–wake and circadian dysfunction early, even prior to the development of AD pathology, might prevent or slow the development of subsequent pathology and later dementia. In Part I of this review, we will discuss the considerable evidence implicating sleep–wake disturbances in the development of Aβ pathology and pathogenesis of AD. In Part II, we will discuss the circadian clock and its function in AD and then postulate on the possible mechanisms by which circadian clock dysfunction could also contribute to AD. Finally, we will conclude with a brief discussion of the therapeutic implications of these findings.

Part I: the sleep–wake cycle as a modulator of ad pathogenesis

Sleep disturbances are a common feature of AD and are observed in mouse AD models

Disorders of the sleep–wake cycle are a common and debilitating symptom of AD. Sleep–wake disruption tends to occur in the moderate stages of the disease and can be multifaceted. The details of sleep disturbances in AD has been extensively reviewed elsewhere and is beyond the scope of this review.1, 2 Briefly, AD patients exhibit disturbances in the timing and duration of the sleep cycle, primarily manifested as increased wakefulness at night (due to increased sleep latency and increased nocturnal awakenings) and increased sleep during the day, which can progress to a loss of day–night variation.3 In terms of sleep architecture, the duration of rapid eye movement (REM) sleep bouts is decreased in AD patients compared with age-matched controls, leading to a cumulative lack of REM sleep.4, 5 Slow-wave sleep is also diminished in AD.6, 7 These changes can occur very early in the course of the disease. Several studies in transgenic mice demonstrate that amyloid deposition in the brain (and in some cases tau aggregation) leads to the disruption of normal sleep architecture, an effect that often precedes the appearance of amyloid plaques. Abnormalities in mice include a loss of robust day–night oscillation in brain lactate,8 phase delay,9 altered nocturnal activity level,10 and decreased non-REM sleep.11, 12, 13 In some cases, sleep abnormalities were reversed by Aβ immunotherapy, demonstrating a causative role for Aβ.8, 13 Thus, Aβ causes sleep disturbances in mice that recapitulate some aspects of human AD.

Sleep disturbances may predict dementia and Aβ pathology

Recently, accumulating evidence has suggested that sleep disturbances may be early indicators of dementia and Aβ pathology and may actually precede the onset of cognitive symptoms in AD. Self-reported sleep problems, as assessed by composite surveys of sleep-related symptoms, have been associated with an increased future risk of developing dementia within 1–9 years.15, 16, 17 Objective sleep–wake data can be obtained using actigraphy, a method in which research subjects wear a small sensor that monitors movement. Although actigraphy measures behavioral activity, it has been shown to be a good surrogate marker for sleep when compared with electroencephalogram (EEG) based sleep studies.18, 19 Fragmentation of sleep, which can be quantified using actigraphic data to analyze the frequency and duration of runs of uninterrupted rest during the night, may be an indicator or arbiter of cognitive impairment.20, 21 Rest fragmentation at night increases with age and is associated with a 1.5-fold increased risk of developing dementia in the ensuing 6-year follow-up period.21 In these studies, total rest time also decreased with age but was not associated with cognitive decline, suggesting that rest-activity fragmentation has a more substantial impact on cognition. Accordingly, a prospective study showed that increased rest fragmentation at night appeared to exacerbate the effects of apolipoprotein E4 on dementia risk, amyloid plaque burden, and tau pathology.22 These studies suggest that alterations in the rest-activity pattern, particularly fragmentation of nighttime rest (a representation of poor sleep consolidation) might portend future development of dementia or even cerebral amyloid-beta (Aβ) deposition. Thus, several studies have examined the relationship between sleep quality and Aβ pathology in cognitively normal older adults. Using actigraphy to assess sleep parameters, Ju et al.23 found that cognitively normal adults with cerebral Aβ deposition on Pittsburgh Compound B (PiB) amyloid positron emission tomography (PET) (PiB) imaging were more likely to nap frequently and had significantly worse sleep efficiency than those without PiB-positive plaques. Subsequently, Spira et al.24 found that cognitively normal older adults who self-report poor sleep were more likely to have Aβ plaque pathology in the precuneus, as also assessed by PiB PET imaging. In total, these studies suggest that presymptomatic amyloid deposition affects sleep and suggest that specific alterations in sleep or activity parameters might be an early biomarker of impending AD. However, the effect sizes observed in these studies were small, illustrating that sleep alterations in preclinical AD are subtle. The existing literature also generally relies on actigraphy, which shows high correlation with EEG-based sleep studies, but does not provide detailed information about sleep stage or quantitative sleep-EEG data. Future studies using EEG-based sleep analysis are likely to provide deeper insights into the relationship between sleep and Aβ pathology and other aspects of AD pathogenesis.

Control of Aβ levels by the sleep–wake cycle

These studies on sleep and AD raise the possibility that altering sleep quality might impact Aβ deposition. In support of this hypothesis, considerable evidence has emerged linking the sleep–wake cycle with Aβ regulation in the brains of mice and humans. Using in vivo cerebral microdialysis, a method in which brain interstitial fluid (ISF) from brain regions such as the hippocampus can be sampled around the clock in awake, freely moving mice and assayed for Aβ, Kang et al.25 revealed that ISF Aβ levels demonstrate a pronounced diurnal rhythm. When mice were kept in standard 12 h:12 h light:dark conditions, levels of Aβ were highest during the dark phase (when mice tend to be awake) and lowest during the light phase (when mice tend to be asleep). ISF Aβ correlated closely with total minutes awake, and the usual dip in Aβ levels at the onset of the light phase could be delayed if the mice were prevented from sleeping. Experiments in humans employing lumbar catheters to collect cerebrospinal fluid (CSF) around the clock revealed similar diurnal oscillations in CSF Aβ levels as were observed in the ISF of mice, although the phase was delayed by ~6 h, presumably because of the transit time of Aβ from the ISF to the lumbar CSF compartment several feet away.25, 26 Thus, Aβ levels in the brain appear to be closely linked to the sleep–wake cycle, both in mice and humans.

The mechanisms that mediate the diurnal oscillation in Aβ are of critical importance, and they are still under investigation. One hypothesis is that Aβ oscillates as a result of similar oscillations in neuronal activity. ISF Aβ levels are known to be regulated by neuronal activity,27, 28, 29 suggesting that increased neuronal activity during the wake phase might mediate the diurnal increase in Aβ. Slow wave sleep is associated with a period of neuronal hyperpolarization and diminished neuronal firing and might thus be expected to be associated with less Aβ production.30 Loss of slow wave sleep, as seen in AD or sleep deprivation protocols,6 would be expected to result in higher cumulative levels of neuronal activity and greater Aβ production. Accordingly, Roh et al.8 demonstrated that lactate, a marker of neuronal activity,29 also exhibited diurnal oscillation in the ISF in phase with Aβ. As plaque pathology advanced, the diurnal oscillations in both lactate and Aβ became damped, but they were restored when plaques were eliminated following Aβ immunotherapy.8 In humans with autosomal dominant AD, oscillations in CSF Aβ also declined as amyloid plaque pathology became evident (as quantified by PiB PET imaging). This study clearly demonstrates the bidirectional relationship between Aβ and sleep and supports the neuronal activity hypothesis. It also demonstrates that accumulating Aβ pathology can mitigate diurnal oscillations in Aβ and disrupt the sleep–wake cycle.

While sleep might limit Aβ production by tempering neuronal activity, it may also regulate the clearance of Aβ from the brain. Xie et al.31 reported that sleep induces significant increases in the volume of extracellular fluid in the brain and enhances the convective bulk flow of metabolites and proteins out of the brain via the ‘glymphatic’ system, or the brain’s form of lymphatic flow, which is controlled by glia. The clearance of exogenously injected Aβ was significantly faster in sleeping mice than waking ones, suggesting that sleep might regulate extracellular Aβ levels by enhancing the removal of Aβ via glymphatic flow. Although this has not been demonstrated for endogenously derived Aβ, these sleep-related fluxes in bulk flow could also explain diurnal variation in the concentration of other proteins and metabolites, including lactate. Further investigation into these and other mechanisms to explain the diurnal oscillation of Aβ is underway.

Sleep deprivation exacerbates Aβ pathology

A clear implication of this relationship between the sleep–wake cycle and Aβ levels is that altering sleep might also modulate Aβ pathology and potentially AD pathogenesis. Kang et al.25 found that forced sleep deprivation caused a striking increase in the Aβ plaque burden in transgenic mice that express AD-associated mutant forms of human amyloid precursor protein (APP) and presenilin-1 (PS1) and develop Aβ plaques with age. Conversely, treatment with the orexin antagonist almorexant, which increased sleep, decreased plaque burden. A subsequent study using mice that express human APP, PS1, and human Tau transgenes also demonstrated an increase in cortical Aβ plaque burden following chronic sleep deprivation.32 Although both studies showed an increase in Aβ pathology with sleep deprivation, they differ on the role of stress-related glucocorticoids in the process. As stress is known to increase the Aβ plaque burden in transgenic mice in a glucocorticoid-dependent manner,33, 34, 35 Rothman et al.32 reported a two-fold increase in glucocorticoid levels in sleep-deprived mice that correlated with plaque burden and suggested that glucocorticoids have a key role in this model. Kang et al.25 demonstrated that ISF Aβ diurnal oscillations can occur in the presence of a corticotrophin releasing hormone antagonist, suggesting that glucocorticoids are not critical to Aβ oscillations, though they did not directly address the role of glucocorticoids in their sleep deprivation experiments. It is likely that the sleep restriction paradigm and the mouse model used can play a major role in dictating how important glucocorticoids are in sleep deprivation-induced Aβ deposition, as the mouse model employed by Rothman et al.36 was very sensitive to psychological stress. Regardless of the role of glucocorticoids, a human study employing CSF collection via a lumbar catheter in healthy volunteers showed that the CSF Aβ level declines after a night of sleep, but this sleep-induced decline is prevented by sleep deprivation, resulting in a higher morning CSF Aβ level.37 Thus, sleep deprivation appears to exacerbate Aβ pathology in mice, and early data suggest that it might do the same in humans.

Sleep deprivation mediates Aβ-independent neuronal injury

Aside from alterations in Aβ, several studies have demonstrated that sleep deprivation can exacerbate neuronal injury via several mechanisms. For example, sleep deprivation can induce tau phosphorylation, synaptic injury, and impaired learning and memory in mice expressing mutant human tau, APP, and PS1 transgenes.32, 38 In healthy human volunteers, a night of sleep deprivation led to a 20% increase in CSF levels of neuron-specific enolase and SB-100, two markers of neuronal injury, suggesting that even instances of acute sleep deprivation may cause neuronal injury.39 Sleep deprivation has also been shown to cause mitochondrial oxidative stress in wake-promoting neurons of the locus ceruleus, and, if prolonged, it can overwhelm the protective SirT3 signaling and cause the death of these neurons.40 As AD is generally considered a disease of protein aggregation, it is notable that sleep deprivation induces endoplasmic reticulum stress and activates the protective unfolded protein response, a response that wanes with age.41, 42 Thus, sleep deprivation may compromise proteostasis in the aging brain, which could contribute to aggregation of Aβ, tau or other proteins associated with neurodegeneration.

Summary, part I

Recent evidence in mice and humans is consistent with the concept that sleep disturbances are not only a consequence of the disease process in AD but might also precede symptom onset and may drive disease pathology (Figure 1). Aβ levels in ISF and CSF exhibit diurnal oscillation that appears to be linked to the sleep–wake cycle and may be mediated by alterations in neuronal activity and/or bulk protein clearance by the glymphatic system. Sleep deprivation exacerbates Aβ plaque pathology while enhancing sleep by inhibiting orexin signaling attenuates plaque accumulation. Finally, sleep deprivation exerts a variety Aβ-independent effects in the brain that could exacerbate neurodegeneration. Because relatively small alterations in Aβ levels can translate into considerable changes in plaque pathology over a long timeframe,43 chronic mild sleep disturbances throughout life might conceivably facilitate Aβ deposition, setting in motion a feed-forward cycle in which Aβ pathology in turn impairs the sleep wake cycle. Poor sleep could also promote tau phosphorylation and neuronal injury that exacerbate Aβ-induced damage, leading to AD. Further research is needed to elucidate the molecular mechanisms that mediate the effects of sleep on Aβ and other neurodegenerative pathways, to determine the role of sleep in the genesis of AD in humans, and to evaluate sleep parameters as therapeutic and diagnostic targets in AD.

Figure 1
Figure 1

Proposed mechanisms linking sleep deprivation, circadian dysfunction, and AD. Dotted arrows represent hypothetical links.

Part II: circadian dysfunction in ad pathogenesis

Although accumulating evidence points to a strong relationship between the sleep–wake cycle and AD pathogenesis, it is important to consider the potential role of the circadian system in mediating some of the observed effects. In the next section, we will discuss how the circadian clock system regulates the sleep–wake cycle and many other aspects of physiology. Although the circadian clock and the sleep–wake cycle are intimately linked, the circadian system is an independent entity with unique therapeutic targets and considerations.

Circadian rhythms are critical to human health

Circadian rhythms, defined as oscillations with a period of 24 h, are a fundamental component of mammalian physiology. Nearly all terrestrial organisms have circadian clock systems that serve to coordinate physiology with external cues such as the light–dark cycle. The circadian oscillation of the sleep–wake cycle is just one of many circadian processes in the body, and it is directly controlled by the circadian clock in mice and humans. Systemic circadian rhythms, such as those observed in the sleep–wake cycle, hormone secretion, and blood pressure, are controlled by the hypothalamic suprachiasmatic nucleus (SCN). The SCN signals via multiple mechanisms, including the autonomic nervous and hormone release, to synchronize cell-autonomous peripheral clocks throughout the body. The SCN controls circadian secretion of melatonin, the ‘dark’ hormone which promotes sleep onset, and SCN lesions lead to loss of circadian rhythms and an arrhythmic sleep–wake cycle in mice.44

Circadian rhythms in the SCN and in most cells throughout the body are maintained by a core cellular clock transcriptional machinery consisting of the bHLH/PAS transcription factors BMAL1 and CLOCK, which heterodimerize and drive transcription of many genes, including their own negative feedback repressors (PERIOD (Per) and CRYPTOCHROME (Cry) genes), which repress BMAL1/CLOCK-mediated transcription.45 BMAL1/CLOCK-mediated transcription is referred to as the ‘positive limb’ of the circadian clock, while the transcriptional suppressor proteins (Per1-3, Cry1-2) are termed the ‘negative limb’. This core clock machinery is found not only in the SCN but also in almost all cells in the body, including neurons and astrocytes,46, 47 and has been estimated to mediate the circadian transcription of 10–20% of all transcripts in a tissue-specific manner.45, 48 The SCN serves as the master body clock, which synchronizes these peripheral oscillators into coherent whole-organism rhythms that are in synch with external light–dark cues. Importantly, whole-organism circadian rhythms and cell-autonomous circadian oscillations are dissociable, as cells removed from an organism continue to oscillate in culture without SCN input and local deletion of clock genes can result in an arrhythmic organ or cell population within a normally rhythmic animal. Likewise, SCN lesions disrupt the synchronization of whole body rhythms but do not prevent the cellular clock from continuing to function in a given cell.49

The circadian clock serves as a master integrator of cellular metabolism in peripheral tissue, and circadian dysfunction has been implicated in the pathogenesis of aging and several disease states, including atherosclerosis, diabetes, and cancer.50, 51, 52 Accordingly, epidemiological evidence shows that humans with disturbed circadian rhythms because of shift work, jetlag, sleep disorder, or aging have an increased risk of metabolic syndrome, coronary disease, cancer, and other chronic diseases.53

Interplay between sleep–wake cycle and circadian rhythms

The sleep–wake cycle and circadian rhythms are often referred to interchangeably, and many studies examining parameters such as actigraphy do not distinguish between them. Sleep is clearly regulated by the circadian clock, as human sleep patterns follow clear circadian patterns, and deletion of the master clock gene Bmal1 abrogates all circadian function54 leading to a total loss of day–night rhythmicity of sleep.55 However, the timing and duration of sleep are also controlled by other factors, such as sleep pressure and arousal. Sleep deprivation can alter the expression of clock genes and the DNA binding patterns of BMAL1/CLOCK heterodimers, thus altering clock function.56 Moreover, efforts to study sleep and circadian phenomena are often muddled because interventions that disrupt rhythms (such as shifting the light: dark cycle or genetic deletion of clock genes) also disrupt sleep. Thus, the impact of such interventions on both sleep and circadian endpoints should be considered.

Circadian dysfunction is a common symptom of Alzheimer disease

Behavioral circadian rhythms decline in aged mice and humans,57, 58 and dysregulation of systemic circadian rhythms, such as those seen in sleep–wake cycle, activity, and melatonin secretion, is a common symptom of AD dementia.3, 58, 59, 60, 61, 62 Mouse models of AD also show disintegration of circadian rhythms with age.10 Drosophila which overexpress Aβ peptides also show a severely disturbed circadian function, though their central pacemaker appears intact, suggesting a downstream effect of Aβ that has not been defined.63, 64 However, several studies in humans have suggested that dysfunction and degeneration of the SCN lies at the root of the circadian dysfunction in AD.65, 66, 67, 68 Pathological studies in humans reveal a significant loss of critical vasopressin- and vasoactive intestinal peptide-expressing neurons in the SCN in AD, two neuronal populations that are known to maintain the SCN circadian function in mice.66, 67 On a molecular level, expression of Bmal1 and Clock decline in senescent cells69 as well as in aged rodent brain tissues.70 Although impaired circadian rhythms have long been understood as a consequence of AD, several lines of recent evidence suggest a possible causative role for circadian dysfunction in AD pathogenesis. Epidemiological data show that impaired systemic circadian rhythms in cognitively normal adults, as assessed by actigraphy, are a significant risk factor for the future development of AD.71 In this study, actigraphy data was analyzed for circadian parameters rather than sleep parameters, even though the primary data collection method was no different than that used in many studies examining sleep in AD. From a genetic standpoint, three small studies have identified three separate polymorphisms in the Clock gene that have been associated with an increased risk of AD,72, 73, 74 though these studies have not yet been replicated in larger cohorts. Finally, the previously described diurnal oscillations in Aβ could be considered to have a circadian rhythm and could be mediated by upstream influences of the circadian system,25, 26 though this has not yet been determined.

Circadian clock gene deletion causes neuronal injury

Although a little is known about the effect of the circadian clock on Aβ or tau pathology in mammals, recent data from our group demonstrate that disruption of the core circadian clock in the brain might directly facilitate neurodegeneration. We generated mice with a brain-specific deletion of the master clock gene Bmal1 in the cortex and hippocampus but with the SCN spared, thus leaving systemic circadian rhythms and sleep–wake cycle intact in the animal while completely disrupting circadian transcriptional regulation in the rest of the brain.75 Despite normal behavioral rhythms (as assessed by wheel-running actigraphy) and sleep–wake cycle (as assessed by EEG), brain-specific Bmal1 knockout mice developed severe cortical astrogliosis, oxidative damage, and synaptic degeneration. This was associated with impaired circadian transcription of several redox defense genes.75 Decreasing levels of BMAL1 in the brain also exacerbated neurodegeneration caused by the mitochondrial toxin 3-nitropropionic acid. These data suggest that declines in circadian function, as seen in aging or AD, might exacerbate neurodegeneration via decreased BMAL1-mediated transcription. Further studies are needed to examine the regulation of clock genes in AD, the downstream mechanisms mediated by the circadian clock in neurons and glia, and how circadian dysfunction regulates Aβ, tau, and other AD-related pathways.

Summary, part II

Although the investigation of the role of circadian rhythms and clock genes in AD pathogenesis is in its infancy, both human and animal data suggest that circadian dysfunction occurs in AD and may precede symptom onset. Furthermore, the circadian clock may have a key role in regulating the expression of neuroprotective proteins and preventing cerebral oxidative stress and synaptic damage (Figure 1). As a key regulator of the sleep–wake cycle, the circadian clock could also potentially contribute to the regulation of Aβ, though this remains to be seen. As the roles of clock genes in other diseases continue to be elucidated, further inquiry into the function of the circadian clock in both the healthy and diseased brain may yield important insights into AD pathogenesis.

Part III: therapeutic implications

Therapies aimed at improving sleep quality and normalizing rhythms in sleep and behavior would address both the sleep and circadian systems and are thus particularly attractive. Considering the profound protective effect of almorexant on Aβ plaque burden in mice,25 the orexin system is a high-priority target. The recent approval of Suvorexant, the first Food and Drug Administration approved orexin receptor antagonist, provides an excellent opportunity to evaluate orexin-targeted therapeutics on Aβ dynamics and cognitive endpoints in early-stage or presymptomatic AD.

Melatonin and light therapy are two methods of synchronizing the circadian clock and enforcing a consistent diurnal rhythm of sleep and activity, and they have been studied extensively in aging and AD with mixed results. In addition to helping regulate circadian rhythms, melatonin is a versatile endogenous antioxidant,76 and its levels decrease both with age77 and preclinical AD.78 Regular melatonin supplementation may be slightly beneficial in improving cognitive performance of patients with mild cognitive impairment.79 In mice, however, there seems to be mixed data on the efficacy of melatonin supplementation on the reduction of amyloid plaques and other correlates of AD.80, 81, 82, 83

Studies have suggested that melatonin has mixed effects in restoring diurnal rhythm in AD patients. Administration of melatonin at bedtime simulates the body’s natural signal that it is night and exerts direct effects on clock gene expression in the SCN and other tissues. While studies in normal aged individuals suggest improvements in sleep quality with melatonin supplementation,84 studies have shown little, if any, effect on sleep in AD patients,85, 86 suggesting additional factors other than endogenous melatonin levels may be affected by AD pathology.

Similarly, ensuring darkness at night and then applying high-intensity white or blue light in the morning has similar effects on the circadian system. Studies have shown that bright-light therapy is effective in improving the stability of diurnal rhythms in dementia patients,87 although this therapy alone may not improve rhythms in AD patients.88 However, bright-light therapy in conjunction with melatonin appears more effective than either therapy alone.88, 89 A specific melatonin M1/M2 receptor agonist (ramelteon) has been approved for insomnia, whereas a second M1/M2 selective receptor agonist (tasimelteon) has been approved for the treatment of circadian disturbance (termed non-24 h sleep–wake disorder) in the blind. Although neither of these agents has been tested in an AD setting, they may achieve higher potency than melatonin itself.

Although still in preclinical development, several groups are designing agents that directly target the circadian clock itself. High throughput screening has identified small molecules that can alter the expression of clock genes and change the amplitude, frequency, and period of circadian oscillations.90, 91 Small molecule agonists of the orphan nuclear receptors Rev-Erbα and -β, which are components of the extended circadian clock, can also exert direct effects on circadian rhythms in mice, leading to improvements in metabolic function.92 Thus, direct targeting of the circadian clock might provide a unique therapeutic opportunity for the treatment of neurodegenerative diseases in the future.

Conclusion

While improving sleep quality and circadian timing could have immediate positive effects on quality of life for AD patients and their caregivers, optimizing these parameters earlier in life might also provide a means of preventing or delaying the development of AD. Understanding the mechanisms by which sleep and circadian disturbances influence the disease process in AD may identify more specific therapeutic targets that could be engaged in healthy middle-aged individuals. Sleep and circadian function could thus represent modifiable risk factors for the future development of AD that are both diagnostically and therapeutically accessible.

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Acknowledgements

The authors received funding from NIH grants P01NS074969 (DMH), PO1-AG03991 (DMH), and K08NS079405 (ESM); the JPB Foundation (DMH), Cure Alzheimer’s Fund (DMH), Alzheimer’s Association grant NIRP-14-305476 (ESM), and Donor’s Cure Foundation (ESM).

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  1. Department of Neurology, Hope Center for Neurological Disorders Research & Knight Alzheimer’s Disease Research Center, Washington University School of Medicine, Saint Louis, MO, USA

    • Erik S Musiek
    • , David D Xiong
    •  & David M Holtzman

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The authors declare no conflict of interest.

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Correspondence to David M Holtzman.

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https://doi.org/10.1038/emm.2014.121

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