Abstract
IL-1β is known promote cyclooxygenase-2 (COX- 2) and matrix metalloproteinase-2 (MMP-2) expression. This study focuses on the characterization of the signaling cascade associated with IL-1β-induced matrix metalloproteinase-2 (MMP- 2) regulation in human chondrocytes. The decrease in collagen levels in the conditioned media was prevented by a broad spectrum MMP inhibitor, suggesting that IL-1β promotes the proteolytic process leading to MMP-2 activation. IL-1β-related MMP-2 expression was found to be dependent on prostaglandin E2 (PGE2) production. In addition, the induction of COX-2 and MMP-2 was inhibited by the pretreatment of chondrocytes with a SB203580 or Ro 31-8220, indicating the involvement of protein kinase C (PKC) or p38 mitogen-activated protein kinase (MAPK). However, there is no cross-talk between PKC and p38 MAPK in the IL-1β-induced MMP-2 activation. Taken together, these results demonstrated that IL-1β induces MMP-2 expression through the PGE2-dependent mechanism in human chondrocytes.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Choi, YA., Lee, DJ., Lim, HK. et al. Interleukin-1β stimulates matrix metalloproteinase-2 expression via a prostaglandin E2-dependent mechanism in human chondrocytes. Exp Mol Med 36, 226–232 (2004). https://doi.org/10.1038/emm.2004.31
Published:
Issue Date:
DOI: https://doi.org/10.1038/emm.2004.31
Keywords
This article is cited by
-
Triumph and tumult of matrix metalloproteinases and their crosstalk with eicosanoids in cancer
Cancer and Metastasis Reviews (2018)