Abstract
The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M ≤ muNa+ ≤ 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex-(ΔT) becomes equal to that of DNA itself (ΔTo). It has been shown that the values of r(b) at which δ ΔT=ΔT-ΔT0=0 depends on ionic strength of a solution. Further increasing of ligand concentration leads to its conversion from stabilizer into the destabilizer of the double-stranded DNA.
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Vardevanyan, P., Antonyan, A., Manukyan, G. et al. Study of ethidium bromide interaction peculiarities with DNA. Exp Mol Med 33, 205–208 (2001). https://doi.org/10.1038/emm.2001.34
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DOI: https://doi.org/10.1038/emm.2001.34