It was reported that membrane fusion was induced by phospholipase D (PLD) in the presence of calcium ion. Initial fusion rate of vesicles in the presence of Ca2+ and PLD was much faster than the expected value when only phosphatidic acid (PA) produced by PLD was taken into account. To elucidate the mechanism of membrane fusion induced by PLD, the interaction of PLD to vesicles and fluorescence changes of PLD were studied. It was found the rate of membrane fusion was much faster than that of membrane aggregation, suggesting PLD did not play a role in vesicle aggregation. The fluorescence of PLD was changed in the presence of vesicle membrane even without Ca2+, indicating that the structural changes of PLD without Ca2+ was not sufficient for membrane fusion. PLD was bound to vesicles irreversibly in the presence of Ca2+ which was essential for induction of membrane fusion. The induction of membrane fusion by PLD may be due to the interaction of PLD itself to vesicles as well as asymmetric distribution of PA on the membranes produced by PLD.
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Park, JB. Interaction of phospholipase D to vesicles induces membrane fusion. Exp Mol Med 28, 141–146 (1996). https://doi.org/10.1038/emm.1996.22
- phospholipase D
- membrane fusion