Abstract
Cardiac troponin-l (cTn-l) specific monoclonal antibodies (McAb) were produced against bovine cTn-l. Six somatic cell fusions using polyethylene glycol (40%) between spleen cells from cTn-l immunized mice BALB/c-NZB F1 hybrid and a mouse myeloma cell line (P3.X63.Ag8.653) were carried out. Sixteen positive monoclonal cell lines were produced; two of them, ID12 and 5F4, showing high specificity for cTn-l. Both of these McAbs were characterized for their antigen specificity, antibody titre in the culture supernatant as well as ascites, class, subclass and light chain analysis. Tissue specificity and species crossreactivity for cTn-l of 5F4 and 1D12 McAb were evaluated and confirmed by enzyme linked immunosorbent assay (ELISA) and immunoblotting. Direct competition ELISA revealed their partial competition for the same epitope on cTn-l. The specificity for ID12 and 5F4 against cTn-l was further confirmed by the reversal action for the actomyosin ATPase inhibition in the presence of these McAbs.
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Haider, K., Stimson, W. Bovine cardiac troponin-I specific monoclonal antibodies which show species cross reactivity. Exp Mol Med 28, 71–76 (1996). https://doi.org/10.1038/emm.1996.11
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DOI: https://doi.org/10.1038/emm.1996.11