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Broccoli sprouts reduce oxidative stress in type 2 diabetes: a randomized double-blind clinical trial



In vitro and animal studies have reported that young broccoli sprouts improve oxidative stress status in diabetic condition. The objective of this double-blind, placebo-controlled, randomized clinical trial was to investigate the effects of broccoli sprouts powder (BSP) on some oxidative stress parameters in type 2 diabetes patients.


A total of 81 patients with type 2 diabetes were randomly assigned to one of three treatment groups for 4 weeks. The groups received either 10 g/d BSP (n=27), 5 g/d BSP (n=29) or placebo (n=25). Serum total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), malondialdehyde (MDA) and oxidized low density lipoprotein (LDL) cholesterol were measured at baseline and at 4 weeks after treatment.


In all, 63 patients in three groups were included in the analysis: 10 g/d BSP (n=21), 5 g/d (n=22) and placebo (n=20). After 4 weeks, consumption of BSP resulted in significant decrease in MDA (P=0.001 for treatment effect), oxidized low density lipoprotein cholesterol (P=0.03 for treatment effect), OSI (P=0.001 for treatment effect) and significant increase in TAC (P=0.001 for treatment effect). No effects were found on TOS.


BSP had favorable effects on oxidative stress status in type 2 diabetes patients.

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This study was funded by the Research Institute of Endocrine Sciences, Shahid Beheshti University of Medical Sciences (Tehran, Iran). The authors express appreciation to the participants of this study and would like to acknowledge the assistance given by Dr N Shakeri in data analysis and Ms N Shiva for language editing of the manuscript. None of the authors had any personal or financial conflicts of interest. The project was designed and implemented by PM and ZB. Serum sample were obtained and analyzed by ZB and MH. The data were analyzed and interpreted by ZB. The manuscript was prepared by PM, ZB, FH, SH and FA. PM and FA supervised overall project and approved the final version of the manuscript for submission.

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Correspondence to P Mirmiran.

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Bahadoran, Z., Mirmiran, P., Hosseinpanah, F. et al. Broccoli sprouts reduce oxidative stress in type 2 diabetes: a randomized double-blind clinical trial. Eur J Clin Nutr 65, 972–977 (2011).

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  • broccoli sprouts
  • oxidative stress
  • clinical trial

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