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The time of day and the interval since previous meal are associated with plasma zinc concentrations and affect estimated risk of zinc deficiency in young children in Peru and Ecuador

European Journal of Clinical Nutrition volume 65, pages 184–190 (2011)Cite this article

Subjects

Abstract

Background/Objectives:

Plasma zinc concentration is the preferred biomarker of zinc status, but the time of day and time since previous meals can modify the results. Measuring fasting plasma zinc concentration is not feasible among young children, so adjustments need to be developed for interpreting results. Our objective is to develop correction factors to adjust for the effects of time of day and interval since the previous meal when measuring plasma zinc concentrations of young children.

Subjects/Methods:

We measured plasma zinc concentrations among young Peruvian (n=297) and Ecuadorian (n=466) children, and constructed regression models adjusting for time of day, interval since previous meal and infections.

Results:

Plasma zinc concentrations were positively related to the number of hours since the previous meal in the Peru trial (r=0.22, P<0.0001) and negatively related to the time of day of blood sampling in both Peru (r=−0.24, P<0.0001) and Ecuador (r=−0.18, P<0.001). In multivariate models, plasma zinc concentrations were ∼2 μg per 100 ml less for each hour later in the morning when blood samples were collected, in both populations, and concentrations were ∼1.0 μg per 100 ml greater for every hour since previous meal consumption in Peru. The percentage of children with low plasma zinc concentrations varied according to both these factors.

Conclusions:

The time of day and the interval since the preceding meal should be recorded when measuring plasma zinc concentration and incorporated into the statistical analysis and interpretation when assessing population zinc status.

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Acknowledgements

This research was supported by the Bill and Melinda Gates Foundation (Peru study); US Department of Agriculture, US Agency for International Development Micronutrient Program, the United Nations Children's Fund (UNICEF), Bristol-Myers/Squibb Freedom to Discover Grant and Grupo Farma del Ecuador (Ecuador study).

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Authors and Affiliations

  1. Department of Nutrition, Program in International and Community Nutrition, University of California, Davis, CA, USA

    J E Arsenault, S E Wuehler & K H Brown

  2. Instituto de Investigación Nutricional, La Molina, Lima, Perú

    D L de Romaña & M E Penny

  3. Corporación Ecuatoriana de Biotecnología, Quito, Ecuador

    F Sempértegui

  4. Medical School, Universidad Central, Quito, Ecuador

    F Sempértegui

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  1. J E Arsenault
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  2. S E Wuehler
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  3. D L de Romaña
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  4. M E Penny
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  5. F Sempértegui
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  6. K H Brown
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Corresponding author

Correspondence to K H Brown.

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The authors declare no conflict of interest.

Additional information

Contributors: JEA contributed to the Peru study implementation, conducted the data analyses and wrote the manuscript. SEW and FS contributed to the Ecuador original study design and implementation. DLdR and MEP contributed to the Peru original study design and implementation. KHB designed and obtained funding for both studies. All authors contributed to interpretation of results, and approved the final manuscript.

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Arsenault, J., Wuehler, S., de Romaña, D. et al. The time of day and the interval since previous meal are associated with plasma zinc concentrations and affect estimated risk of zinc deficiency in young children in Peru and Ecuador. Eur J Clin Nutr 65, 184–190 (2011). https://doi.org/10.1038/ejcn.2010.234

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