Understanding of DNA repair mechanisms could advance treatments for cancer and diseases of aging. But reconstituting DNA repair protein complexes from cancerous tissues to study their mechanisms of action is often time-consuming or, in some cases, impossible. A new technique performing dynamic single-molecule analysis directly on nuclear extracts allows rapid mechanistic analysis of mutant proteins from cancer cells, providing previously unseen insights into their mechanisms of action. This new innovative tool, when combined with rapid data analysis, represents a bridge between the study of biochemistry of purified proteins and molecular biology.
This webcast will describe the workflow and benefits of this new technique when combined with the LUMICKS C-Trap instrument, and will highlight a few instructive examples to demonstrate how this technique is accessible to a wide range of scientists.
Learn:
• How to obtain direct evidence of mode of action of DNA repair protein complexes purified from cancer cells
• How to gain real-time, dynamic insights into DNA-protein interactions at single-molecule resolution
• Required steps to go from nuclear extract to a unique understanding of protein complex mechanisms in 2 weeks or less
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This webcast has been produced by LUMICKS, who retails sole responsibility for content. About this content.
Speaker
Bennett Van Houten, Professor, UPMC Hillman Cancer Center
Bennett Van Houten, PhD, Richard M. Cyert Professor of Molecular Oncology, in the Department of Pharmacology and Chemical Biology at the UPMC Hillman Cancer Center, has more than 30 years’ experience leading research in DNA repair and its relationship to diseases. He has published over 270 papers, and 25 book chapters and reviews. His research focuses on the structure and function of proteins that mediate DNA repair and oxidative stress in human disease.
Moderator
Sarah Hiddleston, Nature Research Custom Media
Sarah Hiddleston is a freelance journalist who has worked with Nature Research Custom Media since 2015. Previously, Sarah worked for a decade in Madras (Chennai), India, specialising in health, pharmaceutical and environmental stories. Sarah holds an MA in Investigative Journalism from City University London, an MSc in Political Theory from the London School of Economics, and an undergraduate degree in History from the University of Cambridge, UK.
