Red dye glows in the eyes of adult fruit flies. Credit: Satya Narayan Sahu
Chemists have synthesized a non-toxic organic dye that can bind to actin, an important cellular protein1. The dye-attached protein gives off a bright red emission when exposed to ultraviolet light.
Actin, which plays a vital role in the shape, adhesion and mobility of cells, can be imaged and monitored using the dye, say researchers at the Sambalpur University in Odisha.
The scientists, led by Satya Narayan Sahu, explored the binding and bioimaging potential of the dye in the actin-rich gut, testes and eyes of adult fruit flies and their larvae.
The team, which included researchers at the National Institute of Technology Rourkela, Odisha, found that the dye bound to the actin-rich villi, finger-like projections in the gut of the fruit fly larvae. It did not damage the gut cells or cause any abnormal behaviour. The dye also accumulated in the eyes of adult fruit flies.
The dye emits a strong signal in the pH range 2 to 10, making it suitable to use as an imaging agent in acidic and slightly basic cellular environments. Its efficiency and fluorescence decay rate are identical to commercial dyes used in bioimaging.
Previous studies have shown that disruption in actin’s structure and dynamics may induce cancer, identifying it as a potential therapeutic target. The dye-based imaging of actin could help in the development of anticancer drugs, the researchers say.
