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Soluble ST2 protein inhibits LPS stimulation on monocyte-derived dendritic cells

Cellular & Molecular Immunology volume 9, pages 399–409 (2012)Cite this article

Abstract

ST2 protein is a soluble splicing variant of ST2L protein, which is the receptor for interleukin-33 (IL-33). Previously, we reported that soluble ST2 suppressed the signal transduction of lipopolysaccharide (LPS) and cytokine production in monocytic cells. To investigate whether or not this inhibitory effect occurs in dendritic cells, which are the key players in innate and adaptive immunity, human monocyte-derived dendritic cells were pre-treated with soluble ST2 protein before LPS stimulation. Although soluble ST2 did not attenuate the LPS-induced maturation of dendritic cells, pre-treatment with soluble ST2 suppressed cytokine production and inhibited LPS signaling. Moreover, the proliferation of naive T cells was inhibited significantly by soluble ST2 pre-treatment. IL-33 had little effect on the cytokine production of immature monocyte-derived dendritic cells. Furthermore, soluble ST2 protein was internalized into dendritic cells, suggesting that soluble ST2 protein acts by a noncanonical mechanism other than the sequestration of IL-33.

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Acknowledgements

We thank Dr Morisada Hayakawa, Dr Nobuhiko Kamoshita and Dr Masaki Kashiwada, Jichi Medical University, for the helpful discussions. The authors are grateful to Ms Reiko Izawa and Ms Chihiro Aoki for their excellent technical support and clerical assistance.

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Jichi Medical University, Shimotsuke-shi, Tochigi, Japan

    Akihisa Nagata, Hiroyuki Tamemoto, Hiromi Ohto-Ozaki, Satoshi Ohta, Shin-ichi Tominaga & Ken Yanagisawa

  2. Hematology Division, National Hospital Organization Disaster Medical Center of Japan, Tachikawa-shi, Tokyo, Japan

    Naoki Takezako

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  1. Akihisa Nagata
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Correspondence to Ken Yanagisawa.

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Nagata, A., Takezako, N., Tamemoto, H. et al. Soluble ST2 protein inhibits LPS stimulation on monocyte-derived dendritic cells. Cell Mol Immunol 9, 399–409 (2012). https://doi.org/10.1038/cmi.2012.29

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